Application possibilities of preparative size exclusion chromatography in a HTE workflow

The application possibilities of preparative size exclusion chromatog. for the detailed anal. of polymer analytes is discussed. Using the example of a star-shaped polymer architecture, the possibilities of utilizing fractions obtained from preparative size exclusion chromatog. and their subsequent off-line characterization with a variety of hyphenated analytic techniques are discussed and compared to one another. It was, for instance, possible to obtain an abs. SEC calibration for the investigated star-shaped polymers that showed very good agreement with theor. expected values and values obtained by abs. mol. wt. detn. techniques by analyzing fractions of different mol. wts. of this polymer architecture by MALDI-TOFMS in order obtain the Mp values required for SEC calibrations. Moreover, the star-shaped polymers were investigated by SEC-viscometry in order to obtain their abs. mol. wt. All anal. results are compared to each other and possibilities as well as limitations are discussed. [on SciFinder (R)]