Antibody detection using a FRET-based protein conformational switch

M.V. Golynskiy, W.F. Rurup, M. Merkx

Research output: Contribution to journalArticleAcademicpeer-review

43 Citations (Scopus)

Abstract

No wash, just go: Classical antibody-detection methods rely on heterogeneous detection schemes that involve multiple, time-consuming binding and washing steps. Here we present a new concept to translate the antigen–antibody interaction directly into a readily detectable fluorescent signal by using a single-chain sensor protein and taking advantage of the unique Y-shaped structure common to all antibodies
Original languageEnglish
Pages (from-to)2264-2267
JournalChemBioChem
Volume11
Issue number16
DOIs
Publication statusPublished - 2011

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