Analytical isotachophoresis

F.M. Everaerts, M. Geurts, F.E.P. Mikkers, T.P.E.M. Verheggen

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Abstract

A survey is given of the latest research on isotachophoresis, especially instrumentation aspects and the application of analytical isotachophoresis. A complete scheme for the linear conductivity detector, to be used with the set of micro-sensing electrodes in direct contact with the electrolytes, is given, and a circuit for a potential gradient detector is also shown. Possible applications of analytical isotachophoresis are considered, and isotachopherograms are given in order to show the way in which information can be deduced from the apparatus described. Arbitrarily chosen examples include small anionic and cationic ionic species, amino acids, peptides and proteins. Analyses in water and deuterium oxide are compared in order to demonstrate the differences in these very similar solvents. A new pump for a counter flow of electrolyte is briefly described.
Original languageEnglish
Pages (from-to)129-155
JournalJournal of Chromatography, A
Volume119
Issue number1
DOIs
Publication statusPublished - 1976

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