Analysis of serum purines and pyrimidines by isotachophoresis

F. Oerlemans, T.P.E.M. Verheggen, F.E.P. Mikkers, F.M. Everaerts, C.H.M.M. Bruijn, de

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Abstract

Purine and pyrimidine metabolism receive attention from a rapidly growing number of workers in the field of inborn errors (1), hematology (2), immunology (3) and oncology (4,5). The availability of metabolite profiles of body fluids and cell contents might attribute to a better understanding of mechanisms underlying metabolic disturbances. This enables a more direct approach for both diagnostic and experimental purposes. For identification of purines and pyrimidines thin-layer high voltage electrophoresis and chromatography can be used (6). A more rapid technique involves high performance liquid chromatography (HPLC) and is widely used at present (7,8). An alternative to HPLC for a screening of metabolite profiles might be isotachophoresis (9). This technique has recently been introduced for the separation and identification of muscle nucleotides (10) and urinary purines and pyrimidines (11). An advantage of isotachophoresis as compared to HPLC is its flexibility: buffers can be changed rapidly, no columns need to be equilibrated. In this paper two systems are presented for the separation of a number of purines and pyrimidines in serum: one low-pH system (pH 3.9) for nucleotides and one high-pH system (pH 7.75) for bases and nucleosides.
Original languageEnglish
Title of host publicationPurine Metabolism in Man, III
EditorsA. Rapado, R.W.E. Watts, C.H.M.M. deBruyn
Place of PublicationNew York
PublisherPlenum Press
Pages429-433
VolumeB
ISBN (Print)0306403110
Publication statusPublished - 1980
Event3rd International Symposium on Purine Metabolism in Man - Madrid, Spain
Duration: 1 Jun 19791 Jun 1979

Publication series

NameAdvances in Experimental Medicine and Biology
PublisherSpringer
ISSN (Print)0065-2598

Conference

Conference3rd International Symposium on Purine Metabolism in Man
Country/TerritorySpain
CityMadrid
Period1/06/791/06/79

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