Analysis of Clinical Samples of Pancreatic Cyst's Lesions with A Multi-Analyte Bioelectronic Simot Array Benchmarked Against Ultrasensitive Chemiluminescent Immunoassay

Cecilia Scandurra, Kim Björkström, Mariapia Caputo, Lucia Sarcina, Enrico Genco, Francesco Modena, Fabrizio Antonio Viola, Celestino Brunetti, Zsolt M. Kovács-Vajna, Cinzia Di Franco, Lena Haeberle, Piero Larizza, Maria Teresa Mancini, Ronald Österbacka, William Reeves, Gaetano Scamarcio, May Wheeler, Mario Caironi, Eugenio Cantatore, Fabrizio TorricelliIrene Esposito, Eleonora Macchia (Corresponding author), Luisa Torsi (Corresponding author)

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Pancreatic cancer, ranking as the third factor in cancer-related deaths, necessitates enhanced diagnostic measures through early detection. In response, SiMoT-Single-molecule with a large Transistor multiplexing array, achieving a Technology Readiness Level of 5, is proposed for a timely identification of pancreatic cancer precursor cysts and is benchmarked against the commercially available chemiluminescent immunoassay SIMOA (Single molecule array) SP-X System. A cohort of 39 samples, comprising 33 cyst fluids and 6 blood plasma specimens, undergoes detailed examination with both technologies. The SiMoT array targets oncoproteins MUC1 and CD55, and oncogene KRAS, while the SIMOA SP-X planar technology exclusively focuses on MUC1 and CD55. Employing Principal Component Analysis (PCA) for multivariate data processing, the SiMoT array demonstrates effective discrimination of malignant/pre-invasive high-grade or potentially malignant low-grade pancreatic cysts from benign non-mucinous cysts. Conversely, PCA analysis applied to SIMOA assay reveals less effective differentiation ability among the three cyst classes. Notably, SiMoT unique capability of concurrently analyzing protein and genetic markers with the threshold of one single molecule in 0.1 mL positions it as a comprehensive and reliable diagnostic tool. The electronic response generated by the SiMoT array facilitates direct digital data communication, suggesting potential applications in the development of field-deployable liquid biopsy.

Original languageEnglish
Article number2308141
Pages (from-to)e2308141
Number of pages14
JournalAdvanced Science
Volume11
Issue number27
DOIs
Publication statusPublished - 17 Jul 2024

Bibliographical note

Publisher Copyright:
© 2024 The Authors. Advanced Science published by Wiley-VCH GmbH.

Keywords

  • bioelectronic transistors
  • liquid biopsy
  • multivariate data processing
  • SIMOA-single-molecule-array-
  • SiMoT-single-molecule-with-a-large-transistor
  • single-molecule biosensors
  • Immunoassay/methods
  • Pancreatic Cyst/diagnosis
  • Humans
  • Proto-Oncogene Proteins p21(ras)/genetics
  • Luminescent Measurements/methods
  • Biomarkers, Tumor/genetics
  • Pancreatic Neoplasms/diagnosis
  • Sensitivity and Specificity
  • Principal Component Analysis/methods

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