An 89Zr-HDL PET Tracer Monitors Response to a CSF1R Inhibitor

Christian A. Mason, Susanne Kossatz, Lukas M. Carter, Giacomo Pirovano, Christian Brand, Navjot Guru, Carlos Pérez-Medina, Jason S. Lewis, Willem J.M. Mulder, Thomas Reiner (Corresponding author)

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

The immune function within the tumor microenvironment has become a prominent therapeutic target, with tumor-associated macrophages (TAMs) playing a critical role in immune suppression. We propose an 89Zr-labeled high-density lipoprotein (89Zr-HDL) nanotracer as a means of monitoring response to immunotherapy. Methods: Female MMTV-PyMT mice were treated with pexidartinib, a colony-stimulating factor 1 receptor (CSF1R) inhibitor, to reduce TAM density. The accumulation of 89Zr-HDL within the tumor was assessed using PET/CT imaging and autoradiography, whereas TAM burden was determined using immunofluorescence. Results: A significant reduction in 89Zr-HDL accumulation was observed in PET/CT images, with 2.9% ± 0.3% and 3.7% ± 0.2% injected dose/g for the pexidartinib- and vehicle-treated mice, respectively. This reduction was corroborated ex vivo and correlated with decreased TAM density. Conclusion: These results support the potential use of 89Zr-HDL nanoparticles as a PET tracer to quickly monitor the response to CSF1R inhibitors and other therapeutic strategies targeting TAMs.

Original languageEnglish
Pages (from-to)433-436
Number of pages4
JournalJournal of Nuclear Medicine
Volume61
Issue number3
DOIs
Publication statusPublished - 1 Mar 2020

Keywords

  • CSF1R inhibitor
  • HDL
  • immunotherapy
  • PET/CT imaging
  • tumor-associated macrophages

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