TY - JOUR
T1 - An investigation concerning the stability of a sterically protected cyanopropyl modified silica substrate
AU - Houbenova, A.
AU - Claessens, H.A.
AU - Haan, de, J.W.
AU - Cramers, C.A.
AU - Stulik, K.
PY - 1994
Y1 - 1994
N2 - The hydrolytic stability of a sterically protected cyanopropyl modified silica phase, used in reversed-phase mode, was studied. This Zorbax SB-CN stationary phase shows superior hydrolytic stability in comparison with conventional CN stationary phases against intensive flushing with aggressive eluents of high and low pH, plain and also with the addn. of an org. modifier. In addn. to chromatog. and elemental anal., solid-state NMR was also used to characterize the changes in the properties of these phases. Also, the effect of sample vol. and nature of the sample solvent on overloading of the SB-CN columns was studied. Vol. overloading was already obsd., when 10 mL of the sample solved in pure org. modifier was injected on the column. However, if the eluent was used as the sample solvent, no significant loss of column performance could be obsd., at least up to 50 mL of sample injections
AB - The hydrolytic stability of a sterically protected cyanopropyl modified silica phase, used in reversed-phase mode, was studied. This Zorbax SB-CN stationary phase shows superior hydrolytic stability in comparison with conventional CN stationary phases against intensive flushing with aggressive eluents of high and low pH, plain and also with the addn. of an org. modifier. In addn. to chromatog. and elemental anal., solid-state NMR was also used to characterize the changes in the properties of these phases. Also, the effect of sample vol. and nature of the sample solvent on overloading of the SB-CN columns was studied. Vol. overloading was already obsd., when 10 mL of the sample solved in pure org. modifier was injected on the column. However, if the eluent was used as the sample solvent, no significant loss of column performance could be obsd., at least up to 50 mL of sample injections
U2 - 10.1080/10826079408013435
DO - 10.1080/10826079408013435
M3 - Article
SN - 0148-3919
VL - 17
SP - 49
EP - 68
JO - Journal of Liquid Chromatography
JF - Journal of Liquid Chromatography
IS - 1
ER -