An aptamer-based new method for competitive fluorescence detection of exosomes

Xiaocheng Yu, Lei He, Myima Pentok, Haowen Yang, Yale Yang, Zhiyang Li, Nongyue He (Corresponding author), Yan Deng (Corresponding author), Song Li, Tonghua Liu (Corresponding author), Xiangyu Chen, Huiwen Luo

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Abstract

Exosomes have been recognized as promising sources of biomarkers for early cancer diagnosis due to their important role in the occurrence and metastasis of cancer, and so the development of a sensitive low-cost detection method for exosomes is highly desirable. In this paper, we report a fluorescence method for the competitive detection of exosomes based on an aptamer specific to CD63 (an exosome transmembrane protein). Aptamer-modified magnetic beads were hybridized with a Cy3-labeled short sequence complementary to a region of the aptamer. In the presence of exosomes, the CD63 on the exosomes bound to the aptamer, resulting in the shedding of the short sequence into the supernatant. The quantity of the exosomes could be estimated by detecting the fluorescence intensity in the supernatant. This method could detect exosomes at a concentration as low as 1.0 × 105 particles per μL under optimal conditions, and the feasibility of the method for exosome detection in complex clinical samples was also proved using simulated serum samples. The detection cost and difficulty are significantly reduced compared to conventional methods, while ensuring sensitivity, and so this method provides a basis for subsequent exosome detection in specific cancer cells.

Original languageEnglish
Pages (from-to)15589-15595
Number of pages7
JournalNanoscale
Volume11
Issue number33
DOIs
Publication statusPublished - 7 Sep 2019

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Fluorescence
Biomarkers
Costs
Cells
Proteins

Cite this

Yu, X., He, L., Pentok, M., Yang, H., Yang, Y., Li, Z., ... Luo, H. (2019). An aptamer-based new method for competitive fluorescence detection of exosomes. Nanoscale, 11(33), 15589-15595. https://doi.org/10.1039/c9nr04050a
Yu, Xiaocheng ; He, Lei ; Pentok, Myima ; Yang, Haowen ; Yang, Yale ; Li, Zhiyang ; He, Nongyue ; Deng, Yan ; Li, Song ; Liu, Tonghua ; Chen, Xiangyu ; Luo, Huiwen. / An aptamer-based new method for competitive fluorescence detection of exosomes. In: Nanoscale. 2019 ; Vol. 11, No. 33. pp. 15589-15595.
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Yu, X, He, L, Pentok, M, Yang, H, Yang, Y, Li, Z, He, N, Deng, Y, Li, S, Liu, T, Chen, X & Luo, H 2019, 'An aptamer-based new method for competitive fluorescence detection of exosomes', Nanoscale, vol. 11, no. 33, pp. 15589-15595. https://doi.org/10.1039/c9nr04050a

An aptamer-based new method for competitive fluorescence detection of exosomes. / Yu, Xiaocheng; He, Lei; Pentok, Myima; Yang, Haowen; Yang, Yale; Li, Zhiyang; He, Nongyue (Corresponding author); Deng, Yan (Corresponding author); Li, Song; Liu, Tonghua (Corresponding author); Chen, Xiangyu; Luo, Huiwen.

In: Nanoscale, Vol. 11, No. 33, 07.09.2019, p. 15589-15595.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - He, Nongyue

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AU - Li, Song

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AU - Luo, Huiwen

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