An actuator chip for in vitro mechano-stimuli responsive studies of primary cortical cell networks

S. Xie, J.G.E. Gardeniers, R. Luttge

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

1 Citation (Scopus)

Abstract

To investigate the hypothesis that dynamic nanoscale stimuli can influence central nerve systems functioning, in this paper we develop a chip based on polydimethylsiloxane (PDMS) soft lithography which exerts nanoscale mechanical loads on an in vitro neuronal network by microfluidic pneumatic deformation of a membrane. Initial analysis on Calcium ion (Ca2+) imaging within rat primary cortical networks shows increased Ca2+activities in the neuronal network after a stimuli was introduced, indicating that the neuronal networks respond to the mechanical stimulus.

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)
Abbreviated titleMicroTAS
CountryIreland
CityDublin
Period9/10/1613/10/16
Internet address

Fingerprint

Polydimethylsiloxane
Microfluidics
Pneumatics
Lithography
Rats
Calcium
Actuators
Membranes
Imaging techniques
Ions

Keywords

  • Actuator chip
  • Caimaging
  • Mechano-stimuli
  • Neuronal networks

Cite this

Xie, S., Gardeniers, J. G. E., & Luttge, R. (2016). An actuator chip for in vitro mechano-stimuli responsive studies of primary cortical cell networks. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 495-496). [M045b] Chemical and Biological Microsystems Society.
Xie, S. ; Gardeniers, J.G.E. ; Luttge, R./ An actuator chip for in vitro mechano-stimuli responsive studies of primary cortical cell networks. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. pp. 495-496
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Xie, S, Gardeniers, JGE & Luttge, R 2016, An actuator chip for in vitro mechano-stimuli responsive studies of primary cortical cell networks. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016., M045b, Chemical and Biological Microsystems Society, pp. 495-496, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016), Dublin, Ireland, 9/10/16.

An actuator chip for in vitro mechano-stimuli responsive studies of primary cortical cell networks. / Xie, S.; Gardeniers, J.G.E.; Luttge, R.

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 495-496 M045b.

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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Xie S, Gardeniers JGE, Luttge R. An actuator chip for in vitro mechano-stimuli responsive studies of primary cortical cell networks. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society. 2016. p. 495-496. M045b.