All-optical imaging of gold nanoparticle geometry using super-resolution microscopy

A. Taylor, R. Verhoef, M. Beuwer, Y. Wang, P. Zijlstra

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Abstract

We demonstrate the all-optical reconstruction of gold nanoparticle geometry using super-resolution microscopy. We employ DNA-PAINT to get exquisite control over the (un)binding kinetics by the number of complementary bases and salt concentration, leading to localization accuracies of ∼5 nm. We employ a dye with an emission spectrum strongly blue-shifted from the plasmon resonance to minimize mislocalization due to plasmon-fluorophore coupling. We correlate the all-optical reconstructions with atomic force microscopy images and find that reconstructed dimensions deviate by no more than ∼10%. Numerical modeling shows that this deviation is determined by the number of events per particle, and the signal-to-background ratio in our measurement. We further find good agreement between the reconstructed orientation and aspect ratio of the particles and single-particle scattering spectroscopy. This method may provide an approach to all-optically image the geometry of single particles in confined spaces such as microfluidic circuits and biological cells, where access with electron beams or tip-based probes is prohibited.

Original languageEnglish
Pages (from-to)2336-2342
Number of pages7
JournalJournal of Physical Chemistry C
Volume122
Issue number4
DOIs
Publication statusPublished - 1 Feb 2018

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Keywords

  • Journal Article

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