Abstract
We demonstrate the all-optical reconstruction of gold nanoparticle geometry using super-resolution microscopy. We employ DNA-PAINT to get exquisite control over the (un)binding kinetics by the number of complementary bases and salt concentration, leading to localization accuracies of ∼5 nm. We employ a dye with an emission spectrum strongly blue-shifted from the plasmon resonance to minimize mislocalization due to plasmon-fluorophore coupling. We correlate the all-optical reconstructions with atomic force microscopy images and find that reconstructed dimensions deviate by no more than ∼10%. Numerical modeling shows that this deviation is determined by the number of events per particle, and the signal-to-background ratio in our measurement. We further find good agreement between the reconstructed orientation and aspect ratio of the particles and single-particle scattering spectroscopy. This method may provide an approach to all-optically image the geometry of single particles in confined spaces such as microfluidic circuits and biological cells, where access with electron beams or tip-based probes is prohibited.
| Original language | English |
|---|---|
| Pages (from-to) | 2336-2342 |
| Number of pages | 7 |
| Journal | Journal of Physical Chemistry C |
| Volume | 122 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1 Feb 2018 |
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- Journal Article
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All-optical imaging of gold nanoparticle geometry using super-resolution microscopy. / Taylor, A.; Verhoef, R.; Beuwer, M.; Wang, Y.; Zijlstra, P.
In: Journal of Physical Chemistry C, Vol. 122, No. 4, 01.02.2018, p. 2336-2342.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - All-optical imaging of gold nanoparticle geometry using super-resolution microscopy
AU - Taylor, A.
AU - Verhoef, R.
AU - Beuwer, M.
AU - Wang, Y.
AU - Zijlstra, P.
PY - 2018/2/1
Y1 - 2018/2/1
N2 - We demonstrate the all-optical reconstruction of gold nanoparticle geometry using super-resolution microscopy. We employ DNA-PAINT to get exquisite control over the (un)binding kinetics by the number of complementary bases and salt concentration, leading to localization accuracies of ∼5 nm. We employ a dye with an emission spectrum strongly blue-shifted from the plasmon resonance to minimize mislocalization due to plasmon-fluorophore coupling. We correlate the all-optical reconstructions with atomic force microscopy images and find that reconstructed dimensions deviate by no more than ∼10%. Numerical modeling shows that this deviation is determined by the number of events per particle, and the signal-to-background ratio in our measurement. We further find good agreement between the reconstructed orientation and aspect ratio of the particles and single-particle scattering spectroscopy. This method may provide an approach to all-optically image the geometry of single particles in confined spaces such as microfluidic circuits and biological cells, where access with electron beams or tip-based probes is prohibited.
AB - We demonstrate the all-optical reconstruction of gold nanoparticle geometry using super-resolution microscopy. We employ DNA-PAINT to get exquisite control over the (un)binding kinetics by the number of complementary bases and salt concentration, leading to localization accuracies of ∼5 nm. We employ a dye with an emission spectrum strongly blue-shifted from the plasmon resonance to minimize mislocalization due to plasmon-fluorophore coupling. We correlate the all-optical reconstructions with atomic force microscopy images and find that reconstructed dimensions deviate by no more than ∼10%. Numerical modeling shows that this deviation is determined by the number of events per particle, and the signal-to-background ratio in our measurement. We further find good agreement between the reconstructed orientation and aspect ratio of the particles and single-particle scattering spectroscopy. This method may provide an approach to all-optically image the geometry of single particles in confined spaces such as microfluidic circuits and biological cells, where access with electron beams or tip-based probes is prohibited.
KW - Journal Article
U2 - 10.1021/acs.jpcc.7b12473
DO - 10.1021/acs.jpcc.7b12473
M3 - Article
C2 - 29422979
VL - 122
SP - 2336
EP - 2342
JO - Journal of Physical Chemistry C
JF - Journal of Physical Chemistry C
SN - 1932-7455
IS - 4
ER -