All-optical imaging of gold nanoparticle geometry using super-resolution microscopy

A. Taylor, R. Verhoef, M. Beuwer, Y. Wang, P. Zijlstra

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)
45 Downloads (Pure)

Abstract

We demonstrate the all-optical reconstruction of gold nanoparticle geometry using super-resolution microscopy. We employ DNA-PAINT to get exquisite control over the (un)binding kinetics by the number of complementary bases and salt concentration, leading to localization accuracies of ∼5 nm. We employ a dye with an emission spectrum strongly blue-shifted from the plasmon resonance to minimize mislocalization due to plasmon-fluorophore coupling. We correlate the all-optical reconstructions with atomic force microscopy images and find that reconstructed dimensions deviate by no more than ∼10%. Numerical modeling shows that this deviation is determined by the number of events per particle, and the signal-to-background ratio in our measurement. We further find good agreement between the reconstructed orientation and aspect ratio of the particles and single-particle scattering spectroscopy. This method may provide an approach to all-optically image the geometry of single particles in confined spaces such as microfluidic circuits and biological cells, where access with electron beams or tip-based probes is prohibited.

Original languageEnglish
Pages (from-to)2336-2342
Number of pages7
JournalJournal of Physical Chemistry C
Volume122
Issue number4
DOIs
Publication statusPublished - 1 Feb 2018

Fingerprint

Gold
Microscopic examination
gold
Nanoparticles
microscopy
Imaging techniques
nanoparticles
Geometry
Fluorophores
geometry
Microfluidics
Aspect ratio
Electron beams
Atomic force microscopy
DNA
Coloring Agents
Dyes
Salts
Spectroscopy
Scattering

Keywords

  • Journal Article

Cite this

@article{9066eef8e4464e3eb6560cb610938a07,
title = "All-optical imaging of gold nanoparticle geometry using super-resolution microscopy",
abstract = "We demonstrate the all-optical reconstruction of gold nanoparticle geometry using super-resolution microscopy. We employ DNA-PAINT to get exquisite control over the (un)binding kinetics by the number of complementary bases and salt concentration, leading to localization accuracies of ∼5 nm. We employ a dye with an emission spectrum strongly blue-shifted from the plasmon resonance to minimize mislocalization due to plasmon-fluorophore coupling. We correlate the all-optical reconstructions with atomic force microscopy images and find that reconstructed dimensions deviate by no more than ∼10{\%}. Numerical modeling shows that this deviation is determined by the number of events per particle, and the signal-to-background ratio in our measurement. We further find good agreement between the reconstructed orientation and aspect ratio of the particles and single-particle scattering spectroscopy. This method may provide an approach to all-optically image the geometry of single particles in confined spaces such as microfluidic circuits and biological cells, where access with electron beams or tip-based probes is prohibited.",
keywords = "Journal Article",
author = "A. Taylor and R. Verhoef and M. Beuwer and Y. Wang and P. Zijlstra",
year = "2018",
month = "2",
day = "1",
doi = "10.1021/acs.jpcc.7b12473",
language = "English",
volume = "122",
pages = "2336--2342",
journal = "Journal of Physical Chemistry C",
issn = "1932-7455",
publisher = "American Chemical Society",
number = "4",

}

All-optical imaging of gold nanoparticle geometry using super-resolution microscopy. / Taylor, A.; Verhoef, R.; Beuwer, M.; Wang, Y.; Zijlstra, P.

In: Journal of Physical Chemistry C, Vol. 122, No. 4, 01.02.2018, p. 2336-2342.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - All-optical imaging of gold nanoparticle geometry using super-resolution microscopy

AU - Taylor, A.

AU - Verhoef, R.

AU - Beuwer, M.

AU - Wang, Y.

AU - Zijlstra, P.

PY - 2018/2/1

Y1 - 2018/2/1

N2 - We demonstrate the all-optical reconstruction of gold nanoparticle geometry using super-resolution microscopy. We employ DNA-PAINT to get exquisite control over the (un)binding kinetics by the number of complementary bases and salt concentration, leading to localization accuracies of ∼5 nm. We employ a dye with an emission spectrum strongly blue-shifted from the plasmon resonance to minimize mislocalization due to plasmon-fluorophore coupling. We correlate the all-optical reconstructions with atomic force microscopy images and find that reconstructed dimensions deviate by no more than ∼10%. Numerical modeling shows that this deviation is determined by the number of events per particle, and the signal-to-background ratio in our measurement. We further find good agreement between the reconstructed orientation and aspect ratio of the particles and single-particle scattering spectroscopy. This method may provide an approach to all-optically image the geometry of single particles in confined spaces such as microfluidic circuits and biological cells, where access with electron beams or tip-based probes is prohibited.

AB - We demonstrate the all-optical reconstruction of gold nanoparticle geometry using super-resolution microscopy. We employ DNA-PAINT to get exquisite control over the (un)binding kinetics by the number of complementary bases and salt concentration, leading to localization accuracies of ∼5 nm. We employ a dye with an emission spectrum strongly blue-shifted from the plasmon resonance to minimize mislocalization due to plasmon-fluorophore coupling. We correlate the all-optical reconstructions with atomic force microscopy images and find that reconstructed dimensions deviate by no more than ∼10%. Numerical modeling shows that this deviation is determined by the number of events per particle, and the signal-to-background ratio in our measurement. We further find good agreement between the reconstructed orientation and aspect ratio of the particles and single-particle scattering spectroscopy. This method may provide an approach to all-optically image the geometry of single particles in confined spaces such as microfluidic circuits and biological cells, where access with electron beams or tip-based probes is prohibited.

KW - Journal Article

U2 - 10.1021/acs.jpcc.7b12473

DO - 10.1021/acs.jpcc.7b12473

M3 - Article

C2 - 29422979

VL - 122

SP - 2336

EP - 2342

JO - Journal of Physical Chemistry C

JF - Journal of Physical Chemistry C

SN - 1932-7455

IS - 4

ER -