Age-dependent changes in geometry, tissue composition and mechanical properties of fetal to adult cryopreserved human heart valves

D. Van Geemen, A.L.F. Soares, P.J.A. Oomen, A. Driessen-Mol, W.J.T. Janssen-van den Broek, A.J. Bogaerdt, van den, A.J.J.C. Bogers, M.J.T.H Goumans, F.P.T. Baaijens, C.V.C. Bouten

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Abstract

There is limited information about age-specific structural and functional properties of human heart valves, while this information is key to the development and evaluation of living valve replacements for pediatric and adolescent patients. Here, we present an extended data set of structure-function properties of cryopreserved human pulmonary and aortic heart valves, providing age-specific information for living valve replacements. Tissue composition, morphology, mechanical properties, and maturation of leaflets from 16 pairs of structurally unaffected aortic and pulmonary valves of human donors (fetal-53 years) were analyzed. Interestingly, no major differences were observed between the aortic and pulmonary valves. Valve annulus and leaflet dimensions increase throughout life. The typical three-layered leaflet structure is present before birth, but becomes more distinct with age. After birth, cell numbers decrease rapidly, while remaining cells obtain a quiescent phenotype and reside in the ventricularis and spongiosa. With age and maturation-but more pronounced in aortic valves-the matrix shows an increasing amount of collagen and collagen cross-links and a reduction in glycosaminoglycans. These matrix changes correlate with increasing leaflet stiffness with age. Our data provide a new and comprehensive overview of the changes of structure-function properties of fetal to adult human semilunar heart valves that can be used to evaluate and optimize future therapies, such as tissue engineering of heart valves. Changing hemodynamic conditions with age can explain initial changes in matrix composition and consequent mechanical properties, but cannot explain the ongoing changes in valve dimensions and matrix composition at older age.

Original languageEnglish
Article numbere0149020
Pages (from-to)1-20
JournalPLoS ONE
Volume11
Issue number2
DOIs
Publication statusPublished - 11 Feb 2016

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heart valves
Heart Valves
Aortic Valve
mechanical properties
Tissue
Pulmonary Valve
Mechanical properties
Geometry
Chemical analysis
Collagen
Parturition
lungs
Pediatrics
Hemodynamics
Tissue Engineering
Glycosaminoglycans
collagen
Tissue engineering
Cell Count
Stiffness

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Van Geemen, D., Soares, A. L. F., Oomen, P. J. A., Driessen-Mol, A., Janssen-van den Broek, W. J. T., Bogaerdt, van den, A. J., ... Bouten, C. V. C. (2016). Age-dependent changes in geometry, tissue composition and mechanical properties of fetal to adult cryopreserved human heart valves. PLoS ONE, 11(2), 1-20. [e0149020]. https://doi.org/10.1371/journal.pone.0149020
Van Geemen, D. ; Soares, A.L.F. ; Oomen, P.J.A. ; Driessen-Mol, A. ; Janssen-van den Broek, W.J.T. ; Bogaerdt, van den, A.J. ; Bogers, A.J.J.C. ; Goumans, M.J.T.H ; Baaijens, F.P.T. ; Bouten, C.V.C. / Age-dependent changes in geometry, tissue composition and mechanical properties of fetal to adult cryopreserved human heart valves. In: PLoS ONE. 2016 ; Vol. 11, No. 2. pp. 1-20.
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Van Geemen, D, Soares, ALF, Oomen, PJA, Driessen-Mol, A, Janssen-van den Broek, WJT, Bogaerdt, van den, AJ, Bogers, AJJC, Goumans, MJTH, Baaijens, FPT & Bouten, CVC 2016, 'Age-dependent changes in geometry, tissue composition and mechanical properties of fetal to adult cryopreserved human heart valves', PLoS ONE, vol. 11, no. 2, e0149020, pp. 1-20. https://doi.org/10.1371/journal.pone.0149020

Age-dependent changes in geometry, tissue composition and mechanical properties of fetal to adult cryopreserved human heart valves. / Van Geemen, D.; Soares, A.L.F.; Oomen, P.J.A.; Driessen-Mol, A.; Janssen-van den Broek, W.J.T.; Bogaerdt, van den, A.J.; Bogers, A.J.J.C.; Goumans, M.J.T.H; Baaijens, F.P.T.; Bouten, C.V.C.

In: PLoS ONE, Vol. 11, No. 2, e0149020, 11.02.2016, p. 1-20.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Van Geemen, D.

AU - Soares, A.L.F.

AU - Oomen, P.J.A.

AU - Driessen-Mol, A.

AU - Janssen-van den Broek, W.J.T.

AU - Bogaerdt, van den, A.J.

AU - Bogers, A.J.J.C.

AU - Goumans, M.J.T.H

AU - Baaijens, F.P.T.

AU - Bouten, C.V.C.

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AB - There is limited information about age-specific structural and functional properties of human heart valves, while this information is key to the development and evaluation of living valve replacements for pediatric and adolescent patients. Here, we present an extended data set of structure-function properties of cryopreserved human pulmonary and aortic heart valves, providing age-specific information for living valve replacements. Tissue composition, morphology, mechanical properties, and maturation of leaflets from 16 pairs of structurally unaffected aortic and pulmonary valves of human donors (fetal-53 years) were analyzed. Interestingly, no major differences were observed between the aortic and pulmonary valves. Valve annulus and leaflet dimensions increase throughout life. The typical three-layered leaflet structure is present before birth, but becomes more distinct with age. After birth, cell numbers decrease rapidly, while remaining cells obtain a quiescent phenotype and reside in the ventricularis and spongiosa. With age and maturation-but more pronounced in aortic valves-the matrix shows an increasing amount of collagen and collagen cross-links and a reduction in glycosaminoglycans. These matrix changes correlate with increasing leaflet stiffness with age. Our data provide a new and comprehensive overview of the changes of structure-function properties of fetal to adult human semilunar heart valves that can be used to evaluate and optimize future therapies, such as tissue engineering of heart valves. Changing hemodynamic conditions with age can explain initial changes in matrix composition and consequent mechanical properties, but cannot explain the ongoing changes in valve dimensions and matrix composition at older age.

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Van Geemen D, Soares ALF, Oomen PJA, Driessen-Mol A, Janssen-van den Broek WJT, Bogaerdt, van den AJ et al. Age-dependent changes in geometry, tissue composition and mechanical properties of fetal to adult cryopreserved human heart valves. PLoS ONE. 2016 Feb 11;11(2):1-20. e0149020. https://doi.org/10.1371/journal.pone.0149020