Advanced CryoTEM and tomography for two- And three-dimensional nano-characterisation of soft matter

F. De Haas; E. Sourty; P.M. Frederik; M.R.J. Vos; N.A.J.M. Sommerdijk; J. Loos; G. De With; D. J. Stokes

Advanced CryoTEM and tomography for two- And three-dimensional nano-characterisation of soft matter

F. De Haas
, E. Sourty
, P.M. Frederik
, M.R.J. Vos
, N.A.J.M. Sommerdijk
, J. Loos
, G. De With
, D. J. Stokes

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

Recent advances in transmission electron microscopy (TEM) have led to unprecedented automated control over data acquisition for various applications. Improvement of methods for obtaining and reconstructing tilt series projections allow us to create three-dimensional tomographic representations of bulk materials at the nanoscale. We demonstrate the combination of the latest TEM technology and tomography, at cryogenic temperatures, for the observation of soft matter such as polymers, hybrid coatings, engineered proteins and nanoparticles for targeted gene and drug delivery, nanocomposites and biomaterials, in conjunction with stateof-the-art vitrification methods for specimen preparation. Using such advanced Cryo-TEM, we aim to understand the organization of molecules, macromolecules and biomolecules, in order to control the properties and performance of materials and devices.

Original language	English
Title of host publication	2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007, Technical Proceedings
Place of Publication	Cambridge
Publisher	NSTI
Pages	73-76
Number of pages	4
Volume	4
ISBN (Print)	1420063766, 9781420063769
Publication status	Published - 23 Aug 2007
Event	2007 NSTI Nanotechnology Conference and Trade Show (NSTI Nanotech 2007) - Santa Clara, United States Duration: 20 May 2007 → 24 May 2007

Conference

Conference	2007 NSTI Nanotechnology Conference and Trade Show (NSTI Nanotech 2007)
Abbreviated title	NSTI Nanotech 2007
Country/Territory	United States
City	Santa Clara
Period	20/05/07 → 24/05/07

Keywords

Cryo-TEM
Reconstruction
Soft matter
Tomography
Vitrobot

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@inproceedings{83595f7d5be2490d9ccf8e4cafa02dc9,

title = "Advanced CryoTEM and tomography for two- And three-dimensional nano-characterisation of soft matter",

abstract = "Recent advances in transmission electron microscopy (TEM) have led to unprecedented automated control over data acquisition for various applications. Improvement of methods for obtaining and reconstructing tilt series projections allow us to create three-dimensional tomographic representations of bulk materials at the nanoscale. We demonstrate the combination of the latest TEM technology and tomography, at cryogenic temperatures, for the observation of soft matter such as polymers, hybrid coatings, engineered proteins and nanoparticles for targeted gene and drug delivery, nanocomposites and biomaterials, in conjunction with stateof-the-art vitrification methods for specimen preparation. Using such advanced Cryo-TEM, we aim to understand the organization of molecules, macromolecules and biomolecules, in order to control the properties and performance of materials and devices.",

keywords = "Cryo-TEM, Reconstruction, Soft matter, Tomography, Vitrobot",

author = "\{De Haas\}, F. and E. Sourty and P.M. Frederik and M.R.J. Vos and N.A.J.M. Sommerdijk and J. Loos and \{De With\}, G. and Stokes, \{D. J.\}",

year = "2007",

month = aug,

day = "23",

language = "English",

isbn = "1420063766",

volume = "4",

pages = "73--76",

booktitle = "2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007, Technical Proceedings",

publisher = "NSTI",

note = "2007 NSTI Nanotechnology Conference and Trade Show (NSTI Nanotech 2007), NSTI Nanotech 2007 ; Conference date: 20-05-2007 Through 24-05-2007",

}

De Haas, F, Sourty, E, Frederik, PM, Vos, MRJ, Sommerdijk, NAJM, Loos, J, De With, G & Stokes, DJ 2007, Advanced CryoTEM and tomography for two- And three-dimensional nano-characterisation of soft matter. in 2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007, Technical Proceedings. vol. 4, NSTI, Cambridge, pp. 73-76, 2007 NSTI Nanotechnology Conference and Trade Show (NSTI Nanotech 2007), Santa Clara, California, United States, 20/05/07.

Advanced CryoTEM and tomography for two- And three-dimensional nano-characterisation of soft matter. / De Haas, F.; Sourty, E.; Frederik, P.M. et al.
2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007, Technical Proceedings. Vol. 4 Cambridge: NSTI, 2007. p. 73-76.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Advanced CryoTEM and tomography for two- And three-dimensional nano-characterisation of soft matter

AU - De Haas, F.

AU - Sourty, E.

AU - Frederik, P.M.

AU - Vos, M.R.J.

AU - Sommerdijk, N.A.J.M.

AU - Loos, J.

AU - De With, G.

AU - Stokes, D. J.

PY - 2007/8/23

Y1 - 2007/8/23

N2 - Recent advances in transmission electron microscopy (TEM) have led to unprecedented automated control over data acquisition for various applications. Improvement of methods for obtaining and reconstructing tilt series projections allow us to create three-dimensional tomographic representations of bulk materials at the nanoscale. We demonstrate the combination of the latest TEM technology and tomography, at cryogenic temperatures, for the observation of soft matter such as polymers, hybrid coatings, engineered proteins and nanoparticles for targeted gene and drug delivery, nanocomposites and biomaterials, in conjunction with stateof-the-art vitrification methods for specimen preparation. Using such advanced Cryo-TEM, we aim to understand the organization of molecules, macromolecules and biomolecules, in order to control the properties and performance of materials and devices.

AB - Recent advances in transmission electron microscopy (TEM) have led to unprecedented automated control over data acquisition for various applications. Improvement of methods for obtaining and reconstructing tilt series projections allow us to create three-dimensional tomographic representations of bulk materials at the nanoscale. We demonstrate the combination of the latest TEM technology and tomography, at cryogenic temperatures, for the observation of soft matter such as polymers, hybrid coatings, engineered proteins and nanoparticles for targeted gene and drug delivery, nanocomposites and biomaterials, in conjunction with stateof-the-art vitrification methods for specimen preparation. Using such advanced Cryo-TEM, we aim to understand the organization of molecules, macromolecules and biomolecules, in order to control the properties and performance of materials and devices.

KW - Cryo-TEM

KW - Reconstruction

KW - Soft matter

KW - Tomography

KW - Vitrobot

UR - https://www.scopus.com/pages/publications/34547997930

M3 - Conference contribution

AN - SCOPUS:34547997930

SN - 1420063766

SN - 9781420063769

VL - 4

SP - 73

EP - 76

BT - 2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007, Technical Proceedings

PB - NSTI

CY - Cambridge

T2 - 2007 NSTI Nanotechnology Conference and Trade Show (NSTI Nanotech 2007)

Y2 - 20 May 2007 through 24 May 2007

ER -

Advanced CryoTEM and tomography for two- And three-dimensional nano-characterisation of soft matter

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Keywords

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