TY - JOUR
T1 - Adsorptive membranes for bilirubin removal
AU - Avramescu, M.E.
AU - Sager, W.F.C.
AU - Borneman, Z.
AU - Wessling, M.
PY - 2004/4/25
Y1 - 2004/4/25
N2 - In this study, we employed ethylene vinyl alcohol (EVAL) adsorptive membranes with bovine serum albumin (BSA) as bioligand for affinity supports for bilirubin (BR) retention. Microfiltration membranes were prepared from ternary or quaternary water/(1-octanol)/DMSO/EVAL systems. To obtain active binding sites for BSA, the EVAL membranes were either chemically functionalized in aqueous and organic medium and by plasma dischargement or physically activated by entrapping of active particles. Static BR removal was determined for all EVAL-BSA membranes. BR retentions relevant for human plasma were gained for the mixed adsorber membranes and additionally investigated in the dynamic mode.
AB - In this study, we employed ethylene vinyl alcohol (EVAL) adsorptive membranes with bovine serum albumin (BSA) as bioligand for affinity supports for bilirubin (BR) retention. Microfiltration membranes were prepared from ternary or quaternary water/(1-octanol)/DMSO/EVAL systems. To obtain active binding sites for BSA, the EVAL membranes were either chemically functionalized in aqueous and organic medium and by plasma dischargement or physically activated by entrapping of active particles. Static BR removal was determined for all EVAL-BSA membranes. BR retentions relevant for human plasma were gained for the mixed adsorber membranes and additionally investigated in the dynamic mode.
KW - Adsorptive membranes
KW - Bilirubin
UR - http://www.scopus.com/inward/record.url?scp=1842531410&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2003.12.020
DO - 10.1016/j.jchromb.2003.12.020
M3 - Article
C2 - 15063328
AN - SCOPUS:1842531410
SN - 1570-0232
VL - 803
SP - 215
EP - 223
JO - Journal of Chromatography, B, Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography, B, Analytical Technologies in the Biomedical and Life Sciences
IS - 2
ER -