A rotating molecular ruler : determining nanometer-scale particle-particle distances in an optomagnetic cluster assay

R.W.L. Vliembergen, van, L.J. IJzendoorn, van, M.W.J. Prins

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademic

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Abstract

We investigate a fast and sensitive optomagnetic biomarker detection technology based on magnetic particles. Antibody-coated superparamagnetic particles capture biomarker molecules and form clusters with a biomarker molecule sandwiched between two particles. The particle clusters are actuated using a rotating magnetic field, which induces an oscillating light scattering cross-section (see Fig. 1a). Sub-picomolar biomarker concentrations can be resolved by the light scattering signals [Ranzoni et al, Nanoletters 2011; ACS Nano 2012]. In this paper we report a method to quantify inter-particle distances with nanometer resolution. The light scattering data show high-frequency signal components (see Fig. 1b). Simulations show that high-frequency components hold detailed information about the geometry of the particle clusters, including a strong dependence on the inter-particle distance (see Fig. 1c). We will report the simulation results and experimental data of corresponding model cluster assays.
Original languageEnglish
Title of host publicationPoster presented at the 10th International Conference on the Scientific and Clinical Applications of Magnetic Carriers, 10-14 June 2014, Dresden, Germany
Pages169-169
Publication statusPublished - 2014
Event10th International Conference on the Scientific and Clinical Applications of Magnetic Carriers, June 10-14, 2014, Dresden, Germany - Dresden, Germany
Duration: 10 Jun 201414 Jun 2014

Conference

Conference10th International Conference on the Scientific and Clinical Applications of Magnetic Carriers, June 10-14, 2014, Dresden, Germany
CountryGermany
CityDresden
Period10/06/1414/06/14

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  • Cite this

    Vliembergen, van, R. W. L., IJzendoorn, van, L. J., & Prins, M. W. J. (2014). A rotating molecular ruler : determining nanometer-scale particle-particle distances in an optomagnetic cluster assay. In Poster presented at the 10th International Conference on the Scientific and Clinical Applications of Magnetic Carriers, 10-14 June 2014, Dresden, Germany (pp. 169-169)