A rotating molecular ruler : determination of particle-particle distance in an optomagnetic cluster assay

R.W.L. Vliembergen, van; L.J. IJzendoorn, van; M.W.J. Prins

A rotating molecular ruler : determination of particle-particle distance in an optomagnetic cluster assay

R.W.L. Vliembergen, van
, L.J. IJzendoorn, van
, M.W.J. Prins

Molecular Biosensing

Research output: Contribution to conference › Poster

Abstract

We investigate a fast and sensitive optomagnetic bionanotechnology for biomarker detection. Antibody-coated superparamagnetic particles capture biomarker molecules and form clusters with a biomarker molecule sandwiched between two particles. These particle clusters can be analyzed using a rotating magnetic field which induces an oscillating light scattering cross-section, resolving sub-picomolar biomarker concentrations [Ranzoni et al, Nanoletters 2011; ACS Nano 2012]. In this paper we report a method to quantify the particle-particle distance with nanometer resolution. Simulations of the light scattering by rotating clusters show that the high-frequency components hold detailed information about the geometry of the particle clusters, including a strong dependence on the inter-particle distance. We will report the simulation results and experimental data of model cluster assays.

Original language	English
Publication status	Published - 2013
Event	conference; Dutch meeting on molecular and cellular biophysics, 2013; 2013-09-30; 2013-10-01 - Duration: 30 Sept 2013 → 1 Oct 2013

Conference

Conference	conference; Dutch meeting on molecular and cellular biophysics, 2013; 2013-09-30; 2013-10-01
Period	30/09/13 → 1/10/13
Other	Dutch meeting on molecular and cellular biophysics, 2013

Bibliographical note

Poster presented at the Dutch meeting on molecular and cellular biophysics, 30 September-1 October 2013, Veldhoven, The Netherlands

Cite this

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title = "A rotating molecular ruler : determination of particle-particle distance in an optomagnetic cluster assay",

abstract = "We investigate a fast and sensitive optomagnetic bionanotechnology for biomarker detection. Antibody-coated superparamagnetic particles capture biomarker molecules and form clusters with a biomarker molecule sandwiched between two particles. These particle clusters can be analyzed using a rotating magnetic field which induces an oscillating light scattering cross-section, resolving sub-picomolar biomarker concentrations [Ranzoni et al, Nanoletters 2011; ACS Nano 2012]. In this paper we report a method to quantify the particle-particle distance with nanometer resolution. Simulations of the light scattering by rotating clusters show that the high-frequency components hold detailed information about the geometry of the particle clusters, including a strong dependence on the inter-particle distance. We will report the simulation results and experimental data of model cluster assays.",

author = "\{Vliembergen, van\}, R.W.L. and \{IJzendoorn, van\}, L.J. and M.W.J. Prins",

note = "Poster presented at the Dutch meeting on molecular and cellular biophysics, 30 September-1 October 2013, Veldhoven, The Netherlands; conference; Dutch meeting on molecular and cellular biophysics, 2013; 2013-09-30; 2013-10-01 ; Conference date: 30-09-2013 Through 01-10-2013",

year = "2013",

language = "English",

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TY - CONF

T1 - A rotating molecular ruler : determination of particle-particle distance in an optomagnetic cluster assay

AU - Vliembergen, van, R.W.L.

AU - IJzendoorn, van, L.J.

AU - Prins, M.W.J.

N1 - Poster presented at the Dutch meeting on molecular and cellular biophysics, 30 September-1 October 2013, Veldhoven, The Netherlands

PY - 2013

Y1 - 2013

N2 - We investigate a fast and sensitive optomagnetic bionanotechnology for biomarker detection. Antibody-coated superparamagnetic particles capture biomarker molecules and form clusters with a biomarker molecule sandwiched between two particles. These particle clusters can be analyzed using a rotating magnetic field which induces an oscillating light scattering cross-section, resolving sub-picomolar biomarker concentrations [Ranzoni et al, Nanoletters 2011; ACS Nano 2012]. In this paper we report a method to quantify the particle-particle distance with nanometer resolution. Simulations of the light scattering by rotating clusters show that the high-frequency components hold detailed information about the geometry of the particle clusters, including a strong dependence on the inter-particle distance. We will report the simulation results and experimental data of model cluster assays.

AB - We investigate a fast and sensitive optomagnetic bionanotechnology for biomarker detection. Antibody-coated superparamagnetic particles capture biomarker molecules and form clusters with a biomarker molecule sandwiched between two particles. These particle clusters can be analyzed using a rotating magnetic field which induces an oscillating light scattering cross-section, resolving sub-picomolar biomarker concentrations [Ranzoni et al, Nanoletters 2011; ACS Nano 2012]. In this paper we report a method to quantify the particle-particle distance with nanometer resolution. Simulations of the light scattering by rotating clusters show that the high-frequency components hold detailed information about the geometry of the particle clusters, including a strong dependence on the inter-particle distance. We will report the simulation results and experimental data of model cluster assays.

M3 - Poster

T2 - conference; Dutch meeting on molecular and cellular biophysics, 2013; 2013-09-30; 2013-10-01

Y2 - 30 September 2013 through 1 October 2013

ER -

A rotating molecular ruler : determination of particle-particle distance in an optomagnetic cluster assay

Abstract

Conference

Bibliographical note

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