A Roadmap to Cardiac Tissue-Engineered Construct Preservation: Insights from Cells, Tissues, and Organs

Vasco Sampaio-Pinto (Corresponding author), Jasmijn Janssen, Nino Chirico, Margarida Serra, Paula M. Alves, Pieter A. Doevendans, Ilja K. Voets, Joost P.G. Sluijter, Linda W. van Laake, Alain van Mil (Corresponding author)

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Abstract

Worldwide, over 26 million patients suffer from heart failure (HF). One strategy aspiring to prevent or even to reverse HF is based on the transplantation of cardiac tissue-engineered (cTE) constructs. These patient-specific constructs aim to closely resemble the native myocardium and, upon implantation on the diseased tissue, support and restore cardiac function, thereby preventing the development of HF. However, cTE constructs off-the-shelf availability in the clinical arena critically depends on the development of efficient preservation methodologies. Short- and long-term preservation of cTE constructs would enable transportation and direct availability. Herein, currently available methods, from normothermic- to hypothermic- to cryopreservation, for the preservation of cardiomyocytes, whole-heart, and regenerative materials are reviewed. A theoretical foundation and recommendations for future research on developing cTE construct specific preservation methods are provided. Current research suggests that vitrification can be a promising procedure to ensure long-term cryopreservation of cTE constructs, despite the need of high doses of cytotoxic cryoprotective agents. Instead, short-term cTE construct preservation can be achieved at normothermic or hypothermic temperatures by administration of protective additives. With further tuning of these promising methods, it is anticipated that cTE construct therapy can be brought one step closer to the patient.

Original languageEnglish
Article number2008517
Number of pages24
JournalAdvanced Materials
Volume33
Issue number27
DOIs
Publication statusPublished - 8 Jul 2021

Bibliographical note

Funding Information:
V.S.-P. and J.J. contributed equally to this work. Illustrations in the Graphical Abstract, Figure 1, Figure 3C, Figure 4B and Figure 5 were created with Biorender.com. The work was funded by The Netherlands Heart Foundation (Dekker Senior Clinical Scientist 2019 to L.W.v.L., Grant No. 2019T056); the alliance between Eindhoven University of Technology, Utrecht University and the University Medical Center Utrecht; the European Union (ERC-2014-StG Contract No. 635928); the Dutch Science Foundation (NWO ECHO Grant No. 712.016.002); the project EVICARE (No. 725229) of the European Research Council to J.P.S.; the Gravitation Program ?Materials Driven Regeneration? by the Netherlands Organization for Scientific Research (RegmedXB #024.003.013); and the Marie Sk?odowska-Curie Actions (Grant agreement RESCUE #801540); the Leducq Fondation grant to the Cure-PLaN network; the EU-funded project BRAV? (H2020, ID:874827); the Funda??o para a Ci?ncia e Tecnologia (FCT)-funded project MetaCardio (PTDC/BTMSAL/32566/2017), and iNOVA4Health, UIDB/04462/2020 and UIDP/04462/2020, a program financially supported by FCT/Minist?rio da Ci?ncia, Tecnologia e Ensino Superior, through Portuguese national funds. Note: The article number in ref. [18] was added on July 6, 2021, after initial publication online.

Funding Information:
V.S.‐P. and J.J. contributed equally to this work. Illustrations in the Graphical Abstract, Figure 1, Figure 3C, Figure 4B and Figure 5 were created with Biorender.com. The work was funded by The Netherlands Heart Foundation (Dekker Senior Clinical Scientist 2019 to L.W.v.L., Grant No. 2019T056); the alliance between Eindhoven University of Technology, Utrecht University and the University Medical Center Utrecht; the European Union (ERC‐2014‐StG Contract No. 635928); the Dutch Science Foundation (NWO ECHO Grant No. 712.016.002); the project EVICARE (No. 725229) of the European Research Council to J.P.S.; the Gravitation Program “Materials Driven Regeneration” by the Netherlands Organization for Scientific Research (RegmedXB #024.003.013); and the Marie Skłodowska‐Curie Actions (Grant agreement RESCUE #801540); the Leducq Fondation grant to the Cure‐PLaN network; the EU‐funded project BRAV∃ (H2020, ID:874827); the Fundação para a Ciência e Tecnologia (FCT)‐funded project MetaCardio (PTDC/BTMSAL/32566/2017), and iNOVA4Health, UIDB/04462/2020 and UIDP/04462/2020, a program financially supported by FCT/Ministério da Ciência, Tecnologia e Ensino Superior, through Portuguese national funds.

Publisher Copyright:
© 2021 The Authors. Advanced Materials published by Wiley-VCH GmbH.

Keywords

  • antifreeze proteins
  • cardiac tissue engineering
  • cryopreservation
  • cryoprotective agents
  • heart failure
  • hypothermic and normothermic preservation
  • vitrification

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