A quantitative assay of cortisol in human plasma by high performance liquid chromatography using a selective chemically bonded stationary phase

J.H.M. van den Berg, C.R. Mol, R.S. Deelder, J.H.H. Thijssen

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59 Citations (Scopus)

Abstract

The extraction and subsequent liquid chromatographic analysis of human plasma samples for cortisol is described. Extraction and chromatography are optimized, resulting in a recovery for cortisol of 96% and a detection limit of 1 microgram cortisol in 100 ml plasma. The application of two chemically modified silicas has been evaluated. The specificity of the method was tested by field desorption - mass spectrometry experiments.
Original languageEnglish
Pages (from-to)165-172
JournalClinica Chimica Acta
Volume78
DOIs
Publication statusPublished - 1977

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