TY - JOUR
T1 - A quantitative assay of cortisol in human plasma by high performance liquid chromatography using a selective chemically bonded stationary phase
AU - van den Berg, J.H.M.
AU - Mol, C.R.
AU - Deelder, R.S.
AU - Thijssen, J.H.H.
PY - 1977
Y1 - 1977
N2 - The extraction and subsequent liquid chromatographic analysis of human plasma samples for cortisol is described. Extraction and chromatography are optimized, resulting in a recovery for cortisol of 96% and a detection limit of 1 microgram cortisol in 100 ml plasma. The application of two chemically modified silicas has been evaluated. The specificity of the method was tested by field desorption - mass spectrometry experiments.
AB - The extraction and subsequent liquid chromatographic analysis of human plasma samples for cortisol is described. Extraction and chromatography are optimized, resulting in a recovery for cortisol of 96% and a detection limit of 1 microgram cortisol in 100 ml plasma. The application of two chemically modified silicas has been evaluated. The specificity of the method was tested by field desorption - mass spectrometry experiments.
U2 - 10.1016/0009-8981(77)90303-5
DO - 10.1016/0009-8981(77)90303-5
M3 - Article
VL - 78
SP - 165
EP - 172
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 0009-8981
ER -