A quantitative assay of cortisol in human plasma by high performance liquid chromatography using a selective chemically bonded stationary phase

J.H.M. van den Berg; C.R. Mol; R.S. Deelder; J.H.H. Thijssen

doi:10.1016/0009-8981(77)90303-5

A quantitative assay of cortisol in human plasma by high performance liquid chromatography using a selective chemically bonded stationary phase

J.H.M. van den Berg, C.R. Mol, R.S. Deelder, J.H.H. Thijssen

Research output: Contribution to journal › Article › Academic › peer-review

62 Citations (Scopus)

Abstract

The extraction and subsequent liquid chromatographic analysis of human plasma samples for cortisol is described. Extraction and chromatography are optimized, resulting in a recovery for cortisol of 96% and a detection limit of 1 microgram cortisol in 100 ml plasma. The application of two chemically modified silicas has been evaluated. The specificity of the method was tested by field desorption - mass spectrometry experiments.

Original language	English
Pages (from-to)	165-172
Journal	Clinica Chimica Acta
Volume	78
DOIs	https://doi.org/10.1016/0009-8981(77)90303-5
Publication status	Published - 1977

Access to Document

10.1016/0009-8981(77)90303-5

Cite this

@article{d8497308044c406a8749b10828a7aae2,

title = "A quantitative assay of cortisol in human plasma by high performance liquid chromatography using a selective chemically bonded stationary phase",

abstract = "The extraction and subsequent liquid chromatographic analysis of human plasma samples for cortisol is described. Extraction and chromatography are optimized, resulting in a recovery for cortisol of 96% and a detection limit of 1 microgram cortisol in 100 ml plasma. The application of two chemically modified silicas has been evaluated. The specificity of the method was tested by field desorption - mass spectrometry experiments.",

author = "{van den Berg}, J.H.M. and C.R. Mol and R.S. Deelder and J.H.H. Thijssen",

year = "1977",

doi = "10.1016/0009-8981(77)90303-5",

language = "English",

volume = "78",

pages = "165--172",

journal = "Clinica Chimica Acta",

issn = "0009-8981",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - A quantitative assay of cortisol in human plasma by high performance liquid chromatography using a selective chemically bonded stationary phase

AU - van den Berg, J.H.M.

AU - Mol, C.R.

AU - Deelder, R.S.

AU - Thijssen, J.H.H.

PY - 1977

Y1 - 1977

N2 - The extraction and subsequent liquid chromatographic analysis of human plasma samples for cortisol is described. Extraction and chromatography are optimized, resulting in a recovery for cortisol of 96% and a detection limit of 1 microgram cortisol in 100 ml plasma. The application of two chemically modified silicas has been evaluated. The specificity of the method was tested by field desorption - mass spectrometry experiments.

AB - The extraction and subsequent liquid chromatographic analysis of human plasma samples for cortisol is described. Extraction and chromatography are optimized, resulting in a recovery for cortisol of 96% and a detection limit of 1 microgram cortisol in 100 ml plasma. The application of two chemically modified silicas has been evaluated. The specificity of the method was tested by field desorption - mass spectrometry experiments.

U2 - 10.1016/0009-8981(77)90303-5

DO - 10.1016/0009-8981(77)90303-5

M3 - Article

SN - 0009-8981

VL - 78

SP - 165

EP - 172

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

ER -

A quantitative assay of cortisol in human plasma by high performance liquid chromatography using a selective chemically bonded stationary phase

Abstract

Access to Document

Fingerprint

Cite this