A simple method to generate membrane permeability of polymersomes consisting of a conventional amphiphilic block copolymer, polyethylene glycol-block-polystyrene (PEG-b-PS), was devised. The three block copolymers are self-assembled into spherical objects, and these objects observed by transmission electron microscopy (TEM) show an average diameter in the range of 200-400 nm. The polymersomes constitute of PEG-b-PSBA containing boronic acid moieties as stimulus-responsive centers, and exhibit both pH and sugar-responsive disassembly into molecularly dissolved block copolymers. It is also shown that when mixed in ratios of 30 wt% and lower, the two block copolymers coexist as a mixture in the membrane of the polymersome without experiencing complete phase separation. After the disassembly of the polymersome at pH 12.6 for 1h, the polymersome solution show fluorescence only in the later elution fractions.