A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays

Yan Ni, Bas J.H.M. Rosier, Eva A. van Aalen, Eva T.L. Hanckmann, Lieuwe Biewenga, Anna-Maria Makri Pistikou, Bart Timmermans, Chris Vu, Sophie Roos, Remco Arts, Wentao Li, Tom F.A. de Greef, Marcel M G J van Borren, Frank J.M. van Kuppeveld, Berend-Jan Bosch, Maarten Merkx (Corresponding author)

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Abstract

Heterogeneous immunoassays such as ELISA have become indispensable in modern bioanalysis, yet translation into point-of-care assays is hindered by their dependence on external calibration and multiple washing and incubation steps. Here, we introduce RAPPID (Ratiometric Plug-and-Play Immunodiagnostics), a mix-and-measure homogeneous immunoassay platform that combines highly specific antibody-based detection with a ratiometric bioluminescent readout. The concept entails analyte-induced complementation of split NanoLuc luciferase fragments, photoconjugated to an antibody sandwich pair via protein G adapters. Introduction of a calibrator luciferase provides a robust ratiometric signal that allows direct in-sample calibration and quantitative measurements in complex media such as blood plasma. We developed RAPPID sensors that allow low-picomolar detection of several protein biomarkers, anti-drug antibodies, therapeutic antibodies, and both SARS-CoV-2 spike protein and anti-SARS-CoV-2 antibodies. With its easy-to-implement standardized workflow, RAPPID provides an attractive, fast, and low-cost alternative to traditional immunoassays, in an academic setting, in clinical laboratories, and for point-of-care applications.

Original languageEnglish
Article number4586
Number of pages12
JournalNature Communications
Volume12
Issue number1
DOIs
Publication statusPublished - 28 Jul 2021

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