A pipette-tip based method for seeding cells to droplet microfluidic platforms

Nidhi Sinha, Nikita Subedi, Florian Wimmers, Melf Soennichsen, Jurjen Tel (Corresponding author)

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Amongst various microfluidic platform designs frequently used for cellular analysis, droplet-microfluidics provides a robust tool for isolating and analyzing cells at the single-cell level by eliminating the influence of external factors on the cellular microenvironment. Encapsulation of cells in droplets is dictated by the Poisson distribution as a function of the number of cells present in each droplet and the average number of cells per volume of droplet. Primary cells, especially immune cells, or clinical specimens can be scarce and loss-less encapsulation of cells remains challenging. In this paper, we present a new methodology that uses pipette-tips to load cells to droplet-based microfluidic devices without the significant loss of cells. With various cell types , we demonstrate efficient cell encapsulation in droplets that closely corresponds to the encapsulation efficiency predicted by the Poisson distribution. Our method ensures loss-less loading of cells to microfluidic platforms and can be easily adapted for downstream single cell analysis, e.g., to decode cellular interactions between different cell types.

LanguageEnglish
Article numbere57848
Number of pages10
JournalJournal of Visualized Experiments (JoVE)
Issue number144
DOIs
StatePublished - 11 Feb 2019

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Cloud seeding
Microfluidics
Encapsulation
Poisson distribution
Poisson Distribution
Lab-On-A-Chip Devices
Single-Cell Analysis
Cellular Microenvironment
Microfluidic Analytical Techniques
Cell Size

Cite this

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title = "A pipette-tip based method for seeding cells to droplet microfluidic platforms",
abstract = "Amongst various microfluidic platform designs frequently used for cellular analysis, droplet-microfluidics provides a robust tool for isolating and analyzing cells at the single-cell level by eliminating the influence of external factors on the cellular microenvironment. Encapsulation of cells in droplets is dictated by the Poisson distribution as a function of the number of cells present in each droplet and the average number of cells per volume of droplet. Primary cells, especially immune cells, or clinical specimens can be scarce and loss-less encapsulation of cells remains challenging. In this paper, we present a new methodology that uses pipette-tips to load cells to droplet-based microfluidic devices without the significant loss of cells. With various cell types , we demonstrate efficient cell encapsulation in droplets that closely corresponds to the encapsulation efficiency predicted by the Poisson distribution. Our method ensures loss-less loading of cells to microfluidic platforms and can be easily adapted for downstream single cell analysis, e.g., to decode cellular interactions between different cell types.",
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A pipette-tip based method for seeding cells to droplet microfluidic platforms. / Sinha, Nidhi; Subedi, Nikita; Wimmers, Florian; Soennichsen, Melf; Tel, Jurjen (Corresponding author).

In: Journal of Visualized Experiments (JoVE), No. 144, e57848, 11.02.2019.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - A pipette-tip based method for seeding cells to droplet microfluidic platforms

AU - Sinha,Nidhi

AU - Subedi,Nikita

AU - Wimmers,Florian

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AU - Tel,Jurjen

PY - 2019/2/11

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AB - Amongst various microfluidic platform designs frequently used for cellular analysis, droplet-microfluidics provides a robust tool for isolating and analyzing cells at the single-cell level by eliminating the influence of external factors on the cellular microenvironment. Encapsulation of cells in droplets is dictated by the Poisson distribution as a function of the number of cells present in each droplet and the average number of cells per volume of droplet. Primary cells, especially immune cells, or clinical specimens can be scarce and loss-less encapsulation of cells remains challenging. In this paper, we present a new methodology that uses pipette-tips to load cells to droplet-based microfluidic devices without the significant loss of cells. With various cell types , we demonstrate efficient cell encapsulation in droplets that closely corresponds to the encapsulation efficiency predicted by the Poisson distribution. Our method ensures loss-less loading of cells to microfluidic platforms and can be easily adapted for downstream single cell analysis, e.g., to decode cellular interactions between different cell types.

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