A novel method for high-pressure freezing of adherent cells for frozen hydrated sectioning and CEMOVIS

R. J. Mesman (Corresponding author)

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

With the development of Cryo Electron Microscopy Of Vitreous Sections (CEMOVIS), imaging cells in a close to native state has become a reality. However with the commonly used carriers for high-pressure freezing and cryo-sectioning, adherent grown cells either need to be detached from their substrate. Here a new method is presented for high-pressure freezing adherent growing cells for frozen-hydrated sectioning and CEMOVIS. Cells are cultured on golden grids, containing a carbon coated Formvar film, and frozen on a membrane carrier which provides the grids with the structural support needed to withstand the strain of trimming and cryo-sectioning. This method was successfully tested for the two different types of high-pressure freezers, those using a pressure chamber (HPM010, EMHPF, Wohlwend Compact 01/02, HPM100) and those directly pressurizing the sample (EMPact series).
Original languageEnglish
Pages (from-to)527-530
Number of pages4
JournalJournal of Structural Biology
Volume183
Issue number3
DOIs
Publication statusPublished - Sep 2013
Externally publishedYes

Keywords

  • High-pressure freezing
  • Adherent cells
  • Cryo-sectioning
  • CEMOVIS

Fingerprint

Dive into the research topics of 'A novel method for high-pressure freezing of adherent cells for frozen hydrated sectioning and CEMOVIS'. Together they form a unique fingerprint.

Cite this