A new method for measuring oxidative stress in claudicants during strenuous exercise using free radical derivatives of antipyrine as indicators: a pilot study

S.A.J. Coolen, M.H.W.A. Wijnen, J.C. Reijenga, H.L. Vader, R.M.H. Roumen, F.A. Huf

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Patients with intermittent claudication disease suffer from temporary lack of oxygen in the legs, caused by narrowing of arteries, resulting in ischemia and followed by reperfusion. The degree of oxidative stress present in 16 patients during strenuous exercise was determined using several indicators. Two derivatives of an exogenous marker, antipyrine (AP), (ie, p-hydroxyantipyrine, p-APOH, and o-hydroxyantipyrine, o-APOH), were assayed in plasma using HPLC-tandem-MS. Plasma malondialdehyde (assayed as thiobarb-ituric acid reactive species, TBARS) was also determined. The branchial/ankle blood pressure index (b-a index) was used to assess the severity of intermittent claudication disease, and plasma lactate concentration was also measured as an indicator of the ischemic situation. Plasma TBARS level did not change significantly after exercise. During the ischemic situation as well as during reperfusion, both free radical derivatives of antipyrine increased significantly in plasma (p
Original languageEnglish
Pages (from-to)181-187
JournalAnnals of Clinical and Laboratory Science
Volume32
Issue number2
Publication statusPublished - 2002

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