TY - CHAP
T1 - A microfluidic droplet array system for cell-based drug combination screening
AU - Du, Guan Sheng
AU - Pan, Jian Zhang
AU - Zhao, Shi Ping
AU - Zhu, Ying
AU - den Toonder, Jaap M.J.
AU - Fang, Qun
PY - 2018/4/10
Y1 - 2018/4/10
N2 - In the last few decades, drug combination therapy has been widely applied in oncology and in other complex diseases. Due to its potential advantage of lower drug toxicity and higher therapeutic efficacy, drug combination treatment has been more and more studied in fundamental labs and pharmacy companies. In this chapter, we report cell-based drug combination screening using a microfluidic droplet system based on a sequential operation droplet array (SODA) technique. In this system, an oil-covered two-dimensional droplet array chip was used as the platform for cell culture and analysis. This chip was fixed in an x–y–z translation stage under control of a computer program. A tapered capillary connected with a syringe pump was coupled with the droplet array chip to achieve multiple droplet manipulations including liquid metering, aspirating, depositing, mixing, and transferring. Complex multistep operations for drug combination screening involving long-term cell culture, medium changing, schedule-dependent drug dosage and stimulation, and cell viability testing were achieved in parallel using the present system. The drug consumption for each screening test was substantially decreased to 5 ng–5 μg, corresponding to 10- to 1000-fold reductions compared with traditional drug screening systems with 96- or 384-well plates.
AB - In the last few decades, drug combination therapy has been widely applied in oncology and in other complex diseases. Due to its potential advantage of lower drug toxicity and higher therapeutic efficacy, drug combination treatment has been more and more studied in fundamental labs and pharmacy companies. In this chapter, we report cell-based drug combination screening using a microfluidic droplet system based on a sequential operation droplet array (SODA) technique. In this system, an oil-covered two-dimensional droplet array chip was used as the platform for cell culture and analysis. This chip was fixed in an x–y–z translation stage under control of a computer program. A tapered capillary connected with a syringe pump was coupled with the droplet array chip to achieve multiple droplet manipulations including liquid metering, aspirating, depositing, mixing, and transferring. Complex multistep operations for drug combination screening involving long-term cell culture, medium changing, schedule-dependent drug dosage and stimulation, and cell viability testing were achieved in parallel using the present system. The drug consumption for each screening test was substantially decreased to 5 ng–5 μg, corresponding to 10- to 1000-fold reductions compared with traditional drug screening systems with 96- or 384-well plates.
KW - Cell-based assay
KW - Droplet-based microfluidics
KW - Drug combination
KW - High-throughput screening
KW - Cell Line
KW - Animals
KW - High-Throughput Screening Assays
KW - Drug Evaluation, Preclinical/instrumentation
KW - Tissue Array Analysis/instrumentation
KW - Cell Culture Techniques/instrumentation
KW - Microfluidics/instrumentation
UR - http://www.scopus.com/inward/record.url?scp=85045386968&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-7792-5_16
DO - 10.1007/978-1-4939-7792-5_16
M3 - Chapter
C2 - 29633215
AN - SCOPUS:85045386968
SN - 978-1-4939-7791-8
T3 - Methods in Molecular Biology
SP - 203
EP - 211
BT - Cell-based microarrays : methods and protocols
A2 - Ertl, P.
A2 - Rothbauer, M.
PB - Springer
CY - Dordrecht
ER -