Abstract
In the last few decades, drug combination therapy has been widely applied in oncology and in other complex diseases. Due to its potential advantage of lower drug toxicity and higher therapeutic efficacy, drug combination treatment has been more and more studied in fundamental labs and pharmacy companies. In this chapter, we report cell-based drug combination screening using a microfluidic droplet system based on a sequential operation droplet array (SODA) technique. In this system, an oil-covered two-dimensional droplet array chip was used as the platform for cell culture and analysis. This chip was fixed in an x–y–z translation stage under control of a computer program. A tapered capillary connected with a syringe pump was coupled with the droplet array chip to achieve multiple droplet manipulations including liquid metering, aspirating, depositing, mixing, and transferring. Complex multistep operations for drug combination screening involving long-term cell culture, medium changing, schedule-dependent drug dosage and stimulation, and cell viability testing were achieved in parallel using the present system. The drug consumption for each screening test was substantially decreased to 5 ng–5 μg, corresponding to 10- to 1000-fold reductions compared with traditional drug screening systems with 96- or 384-well plates.
| Original language | English |
|---|---|
| Title of host publication | Cell-based microarrays : methods and protocols |
| Editors | P. Ertl, M. Rothbauer |
| Place of Publication | Dordrecht |
| Publisher | Springer |
| Pages | 203-211 |
| Number of pages | 9 |
| ISBN (Electronic) | 978-1-4939-7792-5 |
| ISBN (Print) | 978-1-4939-7791-8 |
| DOIs | |
| Publication status | Published - 10 Apr 2018 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1771 |
| ISSN (Print) | 1064-3745 |
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Keywords
- Cell-based assay
- Droplet-based microfluidics
- Drug combination
- High-throughput screening
Cite this
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A microfluidic droplet array system for cell-based drug combination screening. / Du, Guan Sheng; Pan, Jian Zhang; Zhao, Shi Ping; Zhu, Ying; den Toonder, Jaap M.J.; Fang, Qun.
Cell-based microarrays : methods and protocols. ed. / P. Ertl; M. Rothbauer. Dordrecht : Springer, 2018. p. 203-211 (Methods in Molecular Biology; Vol. 1771).Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review
TY - CHAP
T1 - A microfluidic droplet array system for cell-based drug combination screening
AU - Du, Guan Sheng
AU - Pan, Jian Zhang
AU - Zhao, Shi Ping
AU - Zhu, Ying
AU - den Toonder, Jaap M.J.
AU - Fang, Qun
PY - 2018/4/10
Y1 - 2018/4/10
N2 - In the last few decades, drug combination therapy has been widely applied in oncology and in other complex diseases. Due to its potential advantage of lower drug toxicity and higher therapeutic efficacy, drug combination treatment has been more and more studied in fundamental labs and pharmacy companies. In this chapter, we report cell-based drug combination screening using a microfluidic droplet system based on a sequential operation droplet array (SODA) technique. In this system, an oil-covered two-dimensional droplet array chip was used as the platform for cell culture and analysis. This chip was fixed in an x–y–z translation stage under control of a computer program. A tapered capillary connected with a syringe pump was coupled with the droplet array chip to achieve multiple droplet manipulations including liquid metering, aspirating, depositing, mixing, and transferring. Complex multistep operations for drug combination screening involving long-term cell culture, medium changing, schedule-dependent drug dosage and stimulation, and cell viability testing were achieved in parallel using the present system. The drug consumption for each screening test was substantially decreased to 5 ng–5 μg, corresponding to 10- to 1000-fold reductions compared with traditional drug screening systems with 96- or 384-well plates.
AB - In the last few decades, drug combination therapy has been widely applied in oncology and in other complex diseases. Due to its potential advantage of lower drug toxicity and higher therapeutic efficacy, drug combination treatment has been more and more studied in fundamental labs and pharmacy companies. In this chapter, we report cell-based drug combination screening using a microfluidic droplet system based on a sequential operation droplet array (SODA) technique. In this system, an oil-covered two-dimensional droplet array chip was used as the platform for cell culture and analysis. This chip was fixed in an x–y–z translation stage under control of a computer program. A tapered capillary connected with a syringe pump was coupled with the droplet array chip to achieve multiple droplet manipulations including liquid metering, aspirating, depositing, mixing, and transferring. Complex multistep operations for drug combination screening involving long-term cell culture, medium changing, schedule-dependent drug dosage and stimulation, and cell viability testing were achieved in parallel using the present system. The drug consumption for each screening test was substantially decreased to 5 ng–5 μg, corresponding to 10- to 1000-fold reductions compared with traditional drug screening systems with 96- or 384-well plates.
KW - Cell-based assay
KW - Droplet-based microfluidics
KW - Drug combination
KW - High-throughput screening
UR - http://www.scopus.com/inward/record.url?scp=85045386968&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-7792-5_16
DO - 10.1007/978-1-4939-7792-5_16
M3 - Chapter
C2 - 29633215
AN - SCOPUS:85045386968
SN - 978-1-4939-7791-8
T3 - Methods in Molecular Biology
SP - 203
EP - 211
BT - Cell-based microarrays : methods and protocols
A2 - Ertl, P.
A2 - Rothbauer, M.
PB - Springer
CY - Dordrecht
ER -