A micro-optofluidic approach towards individualization of dialysis by continuous electrolyte monitoring

M.K. Sharma, A.J.H. Frijns, J.P. Kooman, R. Mandamparambil, D.M.J. Smeulders

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Study: Most outpatient centers use dialysate with fixed electrolyte concentrations. But pre-dialytic serum concentrations of the major electrolytes (Na+, K+ and Ca2+) differ widely between individual patients. This "one-size fits all approach" leads to the occurrence of acute and chronic cardiovascular complications. As the age and additional disorders of the dialysis patient increase in number, a patient specific dialysate is preferable. Electrochemical methods need frequent calibration and are prone to fouling. Optical sensors offer intrinsic electrical safety, miniaturization perspectives, biocompatibility, less fouling and simultaneous real-time measurement of multiple ions. The aim of this work is to develop a fluorescent micro-optofluidic sensor based on photo-induced electron transfer (PET). Methods: The PET principle exploits the selective quenching of molecular fluorescence. Sensors contain a fluorophore, spacer and an ion-specific receptor. The intensity ratio between fluorescence and absorption gives specific ion concentration. As a first step, we have built the proof-of-concept setup for the validity of the optical excitation and collection system (without a PET sensor). A micro-optofluidic device in poly(dimethylsiloxane) is fabricated with integrated optical fibers. The device is used to observe the fluorescence of Rhodamine B solution. Results: The typical results show that the fluorescence signal is dependent both on flow rate and temperature, but with an optimized design and an integrated temperature sensor this effect can be compensated. An embodiment with PET molecules integrated into the device for in-line monitoring of electrolytes is foreseen in the coming months.
LanguageEnglish
Title of host publicationAbstracts of ASAIO 61st Annual Cconference, June 24-27, 2015, Chicago,
PublisherWolters Kluwer
Pages108
Number of pages1
StatePublished - 2015
Event61st Annual Conference of the American Society for Artificial Internal Organs (ASAIO 2015), June 24-27, 2015, Chicago, IL, USA - Hilton Chicago, Chicago, IL, United States
Duration: 24 Jun 201527 Jun 2015

Publication series

NameASAIO journal
ISSN (Print)1058-2916

Conference

Conference61st Annual Conference of the American Society for Artificial Internal Organs (ASAIO 2015), June 24-27, 2015, Chicago, IL, USA
Abbreviated titleASAIO 2015
CountryUnited States
CityChicago, IL
Period24/06/1527/06/15

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Electrolytes
Dialysis
Fluorescence
Electrons
rhodamine B
Dialysis Solutions
Ions
Equipment and Supplies
Miniaturization
Optical Fibers
Temperature
Calibration
Outpatients
Safety
Serum

Cite this

Sharma, M. K., Frijns, A. J. H., Kooman, J. P., Mandamparambil, R., & Smeulders, D. M. J. (2015). A micro-optofluidic approach towards individualization of dialysis by continuous electrolyte monitoring. In Abstracts of ASAIO 61st Annual Cconference, June 24-27, 2015, Chicago, (pp. 108). (ASAIO journal). Wolters Kluwer.
Sharma, M.K. ; Frijns, A.J.H. ; Kooman, J.P. ; Mandamparambil, R. ; Smeulders, D.M.J./ A micro-optofluidic approach towards individualization of dialysis by continuous electrolyte monitoring. Abstracts of ASAIO 61st Annual Cconference, June 24-27, 2015, Chicago,. Wolters Kluwer, 2015. pp. 108 (ASAIO journal).
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Sharma, MK, Frijns, AJH, Kooman, JP, Mandamparambil, R & Smeulders, DMJ 2015, A micro-optofluidic approach towards individualization of dialysis by continuous electrolyte monitoring. in Abstracts of ASAIO 61st Annual Cconference, June 24-27, 2015, Chicago,. ASAIO journal, Wolters Kluwer, pp. 108, 61st Annual Conference of the American Society for Artificial Internal Organs (ASAIO 2015), June 24-27, 2015, Chicago, IL, USA, Chicago, IL, United States, 24/06/15.

A micro-optofluidic approach towards individualization of dialysis by continuous electrolyte monitoring. / Sharma, M.K.; Frijns, A.J.H.; Kooman, J.P.; Mandamparambil, R.; Smeulders, D.M.J.

Abstracts of ASAIO 61st Annual Cconference, June 24-27, 2015, Chicago,. Wolters Kluwer, 2015. p. 108 (ASAIO journal).

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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T1 - A micro-optofluidic approach towards individualization of dialysis by continuous electrolyte monitoring

AU - Sharma,M.K.

AU - Frijns,A.J.H.

AU - Kooman,J.P.

AU - Mandamparambil,R.

AU - Smeulders,D.M.J.

PY - 2015

Y1 - 2015

N2 - Study: Most outpatient centers use dialysate with fixed electrolyte concentrations. But pre-dialytic serum concentrations of the major electrolytes (Na+, K+ and Ca2+) differ widely between individual patients. This "one-size fits all approach" leads to the occurrence of acute and chronic cardiovascular complications. As the age and additional disorders of the dialysis patient increase in number, a patient specific dialysate is preferable. Electrochemical methods need frequent calibration and are prone to fouling. Optical sensors offer intrinsic electrical safety, miniaturization perspectives, biocompatibility, less fouling and simultaneous real-time measurement of multiple ions. The aim of this work is to develop a fluorescent micro-optofluidic sensor based on photo-induced electron transfer (PET). Methods: The PET principle exploits the selective quenching of molecular fluorescence. Sensors contain a fluorophore, spacer and an ion-specific receptor. The intensity ratio between fluorescence and absorption gives specific ion concentration. As a first step, we have built the proof-of-concept setup for the validity of the optical excitation and collection system (without a PET sensor). A micro-optofluidic device in poly(dimethylsiloxane) is fabricated with integrated optical fibers. The device is used to observe the fluorescence of Rhodamine B solution. Results: The typical results show that the fluorescence signal is dependent both on flow rate and temperature, but with an optimized design and an integrated temperature sensor this effect can be compensated. An embodiment with PET molecules integrated into the device for in-line monitoring of electrolytes is foreseen in the coming months.

AB - Study: Most outpatient centers use dialysate with fixed electrolyte concentrations. But pre-dialytic serum concentrations of the major electrolytes (Na+, K+ and Ca2+) differ widely between individual patients. This "one-size fits all approach" leads to the occurrence of acute and chronic cardiovascular complications. As the age and additional disorders of the dialysis patient increase in number, a patient specific dialysate is preferable. Electrochemical methods need frequent calibration and are prone to fouling. Optical sensors offer intrinsic electrical safety, miniaturization perspectives, biocompatibility, less fouling and simultaneous real-time measurement of multiple ions. The aim of this work is to develop a fluorescent micro-optofluidic sensor based on photo-induced electron transfer (PET). Methods: The PET principle exploits the selective quenching of molecular fluorescence. Sensors contain a fluorophore, spacer and an ion-specific receptor. The intensity ratio between fluorescence and absorption gives specific ion concentration. As a first step, we have built the proof-of-concept setup for the validity of the optical excitation and collection system (without a PET sensor). A micro-optofluidic device in poly(dimethylsiloxane) is fabricated with integrated optical fibers. The device is used to observe the fluorescence of Rhodamine B solution. Results: The typical results show that the fluorescence signal is dependent both on flow rate and temperature, but with an optimized design and an integrated temperature sensor this effect can be compensated. An embodiment with PET molecules integrated into the device for in-line monitoring of electrolytes is foreseen in the coming months.

M3 - Conference contribution

T3 - ASAIO journal

SP - 108

BT - Abstracts of ASAIO 61st Annual Cconference, June 24-27, 2015, Chicago,

PB - Wolters Kluwer

ER -

Sharma MK, Frijns AJH, Kooman JP, Mandamparambil R, Smeulders DMJ. A micro-optofluidic approach towards individualization of dialysis by continuous electrolyte monitoring. In Abstracts of ASAIO 61st Annual Cconference, June 24-27, 2015, Chicago,. Wolters Kluwer. 2015. p. 108. (ASAIO journal).