A method for determining the strain-time endurance of cells in planar tissue-engineered constructs subjected to large compressive deformations

Amit Gefen; Bastiaan van Nierop; Dan L. Bader; Cees W. Oomens

doi:10.1115/SBC2008-192322

A method for determining the strain-time endurance of cells in planar tissue-engineered constructs subjected to large compressive deformations

Amit Gefen, Bastiaan van Nierop, Dan L. Bader, Cees W. Oomens

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

The mechanical environment of cells influences their normal growth and function, and may also affect the development of diseases and chronic injuries. Accordingly, there is substantial interest in determining the endurance of cells subjected to controlled mechanical strains for given time periods. A standardized, generic experimental method for determining strain-time thresholds for cell death is so far missing in the literature. In this study, a new experimental method was developed to measure strain-time thresholds of cells in planar tissue-engineered constructs subjected to large compressive strains. The method was applied to measure a strain-time threshold for differentiated C2C12 murine skeletal muscle cells in tissue-engineered bio-artificial muscle (BAM) constructs.

Original language	English
Title of host publication	Proceedings of the ASME Summer Bioengineering Conference, SBC2008
Pages	253-254
Number of pages	2
Edition	PART A
DOIs	https://doi.org/10.1115/SBC2008-192322
Publication status	Published - 1 Dec 2009
Event	10th ASME Summer Bioengineering Conference, SBC 2008 - Marco Island, United States Duration: 25 Jun 2008 → 29 Jun 2008 Conference number: 10

Conference

Conference	10th ASME Summer Bioengineering Conference, SBC 2008
Abbreviated title	SBC 2008
Country/Territory	United States
City	Marco Island
Period	25/06/08 → 29/06/08

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10.1115/SBC2008-192322

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title = "A method for determining the strain-time endurance of cells in planar tissue-engineered constructs subjected to large compressive deformations",

abstract = "The mechanical environment of cells influences their normal growth and function, and may also affect the development of diseases and chronic injuries. Accordingly, there is substantial interest in determining the endurance of cells subjected to controlled mechanical strains for given time periods. A standardized, generic experimental method for determining strain-time thresholds for cell death is so far missing in the literature. In this study, a new experimental method was developed to measure strain-time thresholds of cells in planar tissue-engineered constructs subjected to large compressive strains. The method was applied to measure a strain-time threshold for differentiated C2C12 murine skeletal muscle cells in tissue-engineered bio-artificial muscle (BAM) constructs.",

author = "Amit Gefen and {van Nierop}, Bastiaan and Bader, {Dan L.} and Oomens, {Cees W.}",

year = "2009",

month = dec,

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doi = "10.1115/SBC2008-192322",

language = "English",

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pages = "253--254",

booktitle = "Proceedings of the ASME Summer Bioengineering Conference, SBC2008",

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note = "10th ASME Summer Bioengineering Conference, SBC 2008, SBC 2008 ; Conference date: 25-06-2008 Through 29-06-2008",

}

Gefen, A, van Nierop, B, Bader, DL & Oomens, CW 2009, A method for determining the strain-time endurance of cells in planar tissue-engineered constructs subjected to large compressive deformations. in Proceedings of the ASME Summer Bioengineering Conference, SBC2008. PART A edn, pp. 253-254, 10th ASME Summer Bioengineering Conference, SBC 2008, Marco Island, Florida, United States, 25/06/08. https://doi.org/10.1115/SBC2008-192322

A method for determining the strain-time endurance of cells in planar tissue-engineered constructs subjected to large compressive deformations. / Gefen, Amit; van Nierop, Bastiaan; Bader, Dan L. et al.
Proceedings of the ASME Summer Bioengineering Conference, SBC2008. PART A. ed. 2009. p. 253-254.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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T1 - A method for determining the strain-time endurance of cells in planar tissue-engineered constructs subjected to large compressive deformations

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AU - Oomens, Cees W.

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N2 - The mechanical environment of cells influences their normal growth and function, and may also affect the development of diseases and chronic injuries. Accordingly, there is substantial interest in determining the endurance of cells subjected to controlled mechanical strains for given time periods. A standardized, generic experimental method for determining strain-time thresholds for cell death is so far missing in the literature. In this study, a new experimental method was developed to measure strain-time thresholds of cells in planar tissue-engineered constructs subjected to large compressive strains. The method was applied to measure a strain-time threshold for differentiated C2C12 murine skeletal muscle cells in tissue-engineered bio-artificial muscle (BAM) constructs.

AB - The mechanical environment of cells influences their normal growth and function, and may also affect the development of diseases and chronic injuries. Accordingly, there is substantial interest in determining the endurance of cells subjected to controlled mechanical strains for given time periods. A standardized, generic experimental method for determining strain-time thresholds for cell death is so far missing in the literature. In this study, a new experimental method was developed to measure strain-time thresholds of cells in planar tissue-engineered constructs subjected to large compressive strains. The method was applied to measure a strain-time threshold for differentiated C2C12 murine skeletal muscle cells in tissue-engineered bio-artificial muscle (BAM) constructs.

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ER -

A method for determining the strain-time endurance of cells in planar tissue-engineered constructs subjected to large compressive deformations

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