A key role for galectin-1 in sprouting angiogenesis revealed by novel rationally designed antibodies

Judy R. van Beijnum, Victor L. Thijssen, Tilman Läppchen, Tse J. Wong, Iris Verel, Maurits Engbersen, Iris A. Schulkens, Raffaella Rossin, Holger Grüll, Arjan W. Griffioen (Corresponding author), Patrycja Nowak-Sliwinska

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

Galectins are carbohydrate binding proteins that function in many key cellular processes. We have previously demonstrated that galectins are essential for tumor angiogenesis and their expression is associated with disease progression. Targeting galectins is therefore a potential anti-angiogenic and anti-cancer strategy. Here, we used a rational approach to generate antibodies against a specific member of this conserved protein family, i.e. galectin-1. We characterized two novel mouse monoclonal antibodies that specifically react with galectin-1 in human, mouse and chicken. We demonstrate that these antibodies are excellent tools to study galectin-1 expression and function in a broad array of biological systems. In a potential diagnostic application, radiolabeled antibodies showed specific targeting of galectin-1 positive tumors. In a therapeutic setting, the antibodies inhibited sprouting angiogenesis in vitro and in vivo, underscoring the key function of galectin-1 in this process.

Original languageEnglish
Pages (from-to)824-835
Number of pages13
JournalInternational Journal of Cancer
Volume139
Issue number4
DOIs
Publication statusPublished - 15 Aug 2016

Keywords

  • angiogenesis
  • antibody
  • galectin-1
  • imaging
  • sprouting
  • therapy

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    van Beijnum, J. R., Thijssen, V. L., Läppchen, T., Wong, T. J., Verel, I., Engbersen, M., ... Nowak-Sliwinska, P. (2016). A key role for galectin-1 in sprouting angiogenesis revealed by novel rationally designed antibodies. International Journal of Cancer, 139(4), 824-835. https://doi.org/10.1002/ijc.30131