A High-throughput Microfluidic Rare Cell Enrichment System Based on Dielectrophoresis and Filtering

Gurhan Ozkayar; Yagmur Demircan Yalçin; Ebru Ozgur; Ufuk Gunduz; Haluk Kulah

doi:10.1016/j.protcy.2017.04.076

A High-throughput Microfluidic Rare Cell Enrichment System Based on Dielectrophoresis and Filtering

Gurhan Ozkayar, Yagmur Demircan Yalçin, Ebru Ozgur, Ufuk Gunduz, Haluk Kulah

Research output: Contribution to journal › Conference article › peer-review

Abstract

In this study, a MEMS-based microfluidic device combining DEP-based cell manipulation with size-based filtration for the enrichment of CTCs from blood with high-throughput was developed. Positive-DEP (pDEP) force and the hydrodynamic force have been used to fine-tune the cell movement over the planar electrodes (sliding) at a high flow rate (30 μl/min). While smaller sized RBCs passed through the gaps and were directed to the waste channels, cancer cells (K562 cells) were filtered and directed to the cancer cell outlet. A cell enrichment factor and depletion rate of RBCs were calculated as 1.45 and 60%, respectively.

Original language	English
Pages (from-to)	177-178
Number of pages	2
Journal	Procedia Technology
Volume	27
DOIs	https://doi.org/10.1016/j.protcy.2017.04.076
Publication status	Published - 2017
Externally published	Yes

Bibliographical note

Part of special issue: Biosensors 2016

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.protcy.2017.04.076

Cite this

@article{717743d5059049adb7630b202da0f4bb,

title = "A High-throughput Microfluidic Rare Cell Enrichment System Based on Dielectrophoresis and Filtering",

abstract = "In this study, a MEMS-based microfluidic device combining DEP-based cell manipulation with size-based filtration for the enrichment of CTCs from blood with high-throughput was developed. Positive-DEP (pDEP) force and the hydrodynamic force have been used to fine-tune the cell movement over the planar electrodes (sliding) at a high flow rate (30 μl/min). While smaller sized RBCs passed through the gaps and were directed to the waste channels, cancer cells (K562 cells) were filtered and directed to the cancer cell outlet. A cell enrichment factor and depletion rate of RBCs were calculated as 1.45 and 60%, respectively.",

author = "Gurhan Ozkayar and {Demircan Yal{\c c}in}, Yagmur and Ebru Ozgur and Ufuk Gunduz and Haluk Kulah",

note = "Part of special issue: Biosensors 2016",

year = "2017",

doi = "10.1016/j.protcy.2017.04.076",

language = "English",

volume = "27",

pages = "177--178",

journal = "Procedia Technology",

issn = "2212-0173",

publisher = "Elsevier",

}

TY - JOUR

T1 - A High-throughput Microfluidic Rare Cell Enrichment System Based on Dielectrophoresis and Filtering

AU - Ozkayar, Gurhan

AU - Demircan Yalçin, Yagmur

AU - Ozgur, Ebru

AU - Gunduz, Ufuk

AU - Kulah, Haluk

N1 - Part of special issue: Biosensors 2016

PY - 2017

Y1 - 2017

N2 - In this study, a MEMS-based microfluidic device combining DEP-based cell manipulation with size-based filtration for the enrichment of CTCs from blood with high-throughput was developed. Positive-DEP (pDEP) force and the hydrodynamic force have been used to fine-tune the cell movement over the planar electrodes (sliding) at a high flow rate (30 μl/min). While smaller sized RBCs passed through the gaps and were directed to the waste channels, cancer cells (K562 cells) were filtered and directed to the cancer cell outlet. A cell enrichment factor and depletion rate of RBCs were calculated as 1.45 and 60%, respectively.

AB - In this study, a MEMS-based microfluidic device combining DEP-based cell manipulation with size-based filtration for the enrichment of CTCs from blood with high-throughput was developed. Positive-DEP (pDEP) force and the hydrodynamic force have been used to fine-tune the cell movement over the planar electrodes (sliding) at a high flow rate (30 μl/min). While smaller sized RBCs passed through the gaps and were directed to the waste channels, cancer cells (K562 cells) were filtered and directed to the cancer cell outlet. A cell enrichment factor and depletion rate of RBCs were calculated as 1.45 and 60%, respectively.

U2 - 10.1016/j.protcy.2017.04.076

DO - 10.1016/j.protcy.2017.04.076

M3 - Conference article

VL - 27

SP - 177

EP - 178

JO - Procedia Technology

JF - Procedia Technology

SN - 2212-0173

ER -

A High-throughput Microfluidic Rare Cell Enrichment System Based on Dielectrophoresis and Filtering

Abstract

Bibliographical note

UN SDGs

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