A DEP-based lab-on-a-chip system for the detection of multidrug resistance in K562 leukemia cells

Y. Demircan Yalçın; G. Özkayar; E. Özgür; U. Gündüz; H. Külah

doi:10.31438/trf.hh2016.86

A DEP-based lab-on-a-chip system for the detection of multidrug resistance in K562 leukemia cells

Y. Demircan Yalçın
, G. Özkayar
, E. Özgür
, U. Gündüz
, H. Külah

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

1 Citation (Scopus)

Abstract

This study presents a DEP-based lab-on-a-chip (LOC) system for label-free detection of multidrug resistant (MDR) K562 leukemia cells in a cell mixture, consisting of red blood cells (RBCs) and MDR-K562 cells, for the first time in the literature. The system consists of 2 consecutive DEP units, one for the depletion of RBCs and the other for capturing of MDR-K562 cells. RBCs are depleted by 60% in the first unit. In the second unit, MDR-K562 cell detection is performed with 100% selectivity at a flow rate of 10 μl/min and at 20 V_pp in a cell mixture, containing less than 25% MDR-K562 cells.

Original language	English
Title of host publication	2016 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2016
Editors	Mark G. Allen, Tina Lamers
Publisher	Transducer Research Foundation
Pages	316-319
Number of pages	4
ISBN (Electronic)	9781940470023
DOIs	https://doi.org/10.31438/trf.hh2016.86
Publication status	Published - 1 Jan 2016
Externally published	Yes
Event	2016 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2016 - Hilton Head, United States Duration: 5 Jun 2016 → 9 Jun 2016

Conference

Conference	2016 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2016
Country/Territory	United States
City	Hilton Head
Period	5/06/16 → 9/06/16

Funding

The authors acknowledge The Scientific and Technological Research Council of Turkey (TUBITAK) for the scholarship support for Mrs. Yağmur Demircan Yalçın and for the financial support through project 111E194. *First and second authors have equal contributions on this study.

Access to Document

10.31438/trf.hh2016.86

Cite this

Yalçın, Y. D., Özkayar, G., Özgür, E., Gündüz, U., & Külah, H. (2016). A DEP-based lab-on-a-chip system for the detection of multidrug resistance in K562 leukemia cells. In M. G. Allen, & T. Lamers (Eds.), 2016 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2016 (pp. 316-319). Transducer Research Foundation. https://doi.org/10.31438/trf.hh2016.86

@inproceedings{83e839f5ebc84a508ef895da7467edab,

title = "A DEP-based lab-on-a-chip system for the detection of multidrug resistance in K562 leukemia cells",

abstract = "This study presents a DEP-based lab-on-a-chip (LOC) system for label-free detection of multidrug resistant (MDR) K562 leukemia cells in a cell mixture, consisting of red blood cells (RBCs) and MDR-K562 cells, for the first time in the literature. The system consists of 2 consecutive DEP units, one for the depletion of RBCs and the other for capturing of MDR-K562 cells. RBCs are depleted by 60\% in the first unit. In the second unit, MDR-K562 cell detection is performed with 100\% selectivity at a flow rate of 10 μl/min and at 20 Vpp in a cell mixture, containing less than 25\% MDR-K562 cells.",

author = "Yal{\c c}ın, \{Y. Demircan\} and G. {\"O}zkayar and E. {\"O}zg{\"u}r and U. G{\"u}nd{\"u}z and H. K{\"u}lah",

year = "2016",

month = jan,

day = "1",

doi = "10.31438/trf.hh2016.86",

language = "English",

pages = "316--319",

editor = "Allen, \{Mark G.\} and Tina Lamers",

booktitle = "2016 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2016",

publisher = "Transducer Research Foundation",

note = "2016 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2016 ; Conference date: 05-06-2016 Through 09-06-2016",

}

Yalçın, YD, Özkayar, G, Özgür, E, Gündüz, U & Külah, H 2016, A DEP-based lab-on-a-chip system for the detection of multidrug resistance in K562 leukemia cells. in MG Allen & T Lamers (eds), 2016 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2016. Transducer Research Foundation, pp. 316-319, 2016 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2016, Hilton Head, United States, 5/06/16. https://doi.org/10.31438/trf.hh2016.86

A DEP-based lab-on-a-chip system for the detection of multidrug resistance in K562 leukemia cells. / Yalçın, Y. Demircan; Özkayar, G.; Özgür, E. et al.
2016 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2016. ed. / Mark G. Allen; Tina Lamers. Transducer Research Foundation, 2016. p. 316-319.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - A DEP-based lab-on-a-chip system for the detection of multidrug resistance in K562 leukemia cells

AU - Yalçın, Y. Demircan

AU - Özkayar, G.

AU - Özgür, E.

AU - Gündüz, U.

AU - Külah, H.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - This study presents a DEP-based lab-on-a-chip (LOC) system for label-free detection of multidrug resistant (MDR) K562 leukemia cells in a cell mixture, consisting of red blood cells (RBCs) and MDR-K562 cells, for the first time in the literature. The system consists of 2 consecutive DEP units, one for the depletion of RBCs and the other for capturing of MDR-K562 cells. RBCs are depleted by 60% in the first unit. In the second unit, MDR-K562 cell detection is performed with 100% selectivity at a flow rate of 10 μl/min and at 20 Vpp in a cell mixture, containing less than 25% MDR-K562 cells.

AB - This study presents a DEP-based lab-on-a-chip (LOC) system for label-free detection of multidrug resistant (MDR) K562 leukemia cells in a cell mixture, consisting of red blood cells (RBCs) and MDR-K562 cells, for the first time in the literature. The system consists of 2 consecutive DEP units, one for the depletion of RBCs and the other for capturing of MDR-K562 cells. RBCs are depleted by 60% in the first unit. In the second unit, MDR-K562 cell detection is performed with 100% selectivity at a flow rate of 10 μl/min and at 20 Vpp in a cell mixture, containing less than 25% MDR-K562 cells.

UR - https://www.scopus.com/pages/publications/85071421967

U2 - 10.31438/trf.hh2016.86

DO - 10.31438/trf.hh2016.86

M3 - Conference contribution

AN - SCOPUS:85071421967

SP - 316

EP - 319

BT - 2016 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2016

A2 - Allen, Mark G.

A2 - Lamers, Tina

PB - Transducer Research Foundation

T2 - 2016 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2016

Y2 - 5 June 2016 through 9 June 2016

ER -

A DEP-based lab-on-a-chip system for the detection of multidrug resistance in K562 leukemia cells

Abstract

Conference

Funding

Access to Document

Other files and links

Fingerprint

Cite this