A combinatorial approach towards the design of nanofibrous scaffolds for chondrogenesis

Maqsood Ahmed, Tiago André Da Silva Ramos, Febriyani Damanik, Bach Quang Le, Paul Wieringa, Martin Bennink, Clemens Van Blitterswijk, Jan De Boer, Lorenzo Moroni

Research output: Contribution to journalArticleAcademicpeer-review

16 Citations (Scopus)

Abstract

The extracellular matrix (ECM) is a three-dimensional (3D) structure composed of proteinaceous fibres that provide physical and biological cues to direct cell behaviour. Here, we build a library of hybrid collagen-polymer fibrous scaffolds with nanoscale dimensions and screen them for their ability to grow chondrocytes for cartilage repair. Poly(lactic acid) and poly (lactic-co-glycolic acid) at two different monomer ratios (85:15 and 50:50) were incrementally blended with collagen. Physical properties (wettability and stiffness) of the scaffolds were characterized and related to biological performance (proliferation, ECM production, and gene expression) and structure-function relationships were developed. We found that soft scaffolds with an intermediate wettability composed of the highly biodegradable PLGA50:50 and collagen, in two ratios (40:60 and 60:40), were optimal for chondrogenic differentiation of ATDC5 cells as determined by increased ECM production and enhanced cartilage specific gene expression. Long-term cultures indicated a stable phenotype with minimal de-differentiation or hypertrophy. The combinatorial methodology applied herein is a promising approach for the design and development of scaffolds for regenerative medicine.

Original languageEnglish
Article number14804
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 7 Oct 2015
Externally publishedYes

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