A 3D finite element model of ventral furrow invagination in the Drosophila melanogaster embryo

Vito Conte, José J. Muñoz, Mark Miodownik

Research output: Contribution to journalArticleAcademicpeer-review

54 Citations (Scopus)

Abstract

The paper describes a mechanical model of epithelial tissue development in Drosophila embryos to investigate a buckling phenomenon called invagination. The finite element method is used to model this ventral furrow formation in 3D by decomposing the total deformation into two parts: an imposed active deformation, and an elastic passive deformation superimposed onto the latter. The model imposes as boundary conditions (i) a constant yolk volume and (ii) a sliding contact condition of the cells against the vitelline membrane, which is interpolated as a B-Spline surface. The active deformation simulates the effects of apical constriction and apico-basal elongation of cells. This set of local cellular mechanisms leads to global shape changes of the embryo which are associated with known gene expressions. Using the model we have tested different plausible hypotheses postulated to account for the mechanical behaviour of epithelial tissues. In particular, we conclude that only certain combinations of local cell shape change can successfully reproduce the invagination process. We have quantitatively compared the model with a 2D model and shown that it exhibits a more robust invagination phenomenon. The 3D model has also revealed that invagination causes a yolk flow from the central region to the anterior and posterior ends of the embryo, causing an accordion-like global compression and expansion wave to move through the embryo. Such a phenomenon cannot be described by 2D models.

Original languageEnglish
Pages (from-to)188-198
Number of pages11
JournalJournal of the Mechanical Behavior of Biomedical Materials
Volume1
Issue number2
DOIs
Publication statusPublished - 1 Apr 2008
Externally publishedYes

Keywords

  • Active forces
  • Drosophila
  • Finite elasticity
  • Finite elements
  • Gastrulation
  • Invagination

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