3D-Oxygen Gradient Chip for Cancer Cell Migration Research

Pan Zuo; Jelle J.F. Sleeboom; Jaap M.J. den Toonder

3D-Oxygen Gradient Chip for Cancer Cell Migration Research

Pan Zuo (Corresponding author)
, Jelle J.F. Sleeboom
, Jaap M.J. den Toonder

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

Metastasis, the major cause of cancer related deaths, is a multi-step procedure, influenced by many factors and cues present in the tumor microenvironment. One important but not thoroughly studied factor is hypoxia. In this study, we introduce the design, fabrication and validation of a PDMS chip to study the effect of hypoxia on the migration of cancer cells in 3D. The chip contains a cell culture chamber in which a linear and stable oxygen gradient can be established by an oxygen leaching fluid flowing through a neighboring channel, and enables to observe the migration of cancer cells in 3D.

Original language	English
Title of host publication	MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	514-515
Number of pages	2
ISBN (Electronic)	9781733419048
Publication status	Published - 2022
Event	26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 - Hybrid, Hangzhou, China Duration: 23 Oct 2022 → 27 Oct 2022

Publication series

Name	MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference	26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022
Country/Territory	China
City	Hybrid, Hangzhou
Period	23/10/22 → 27/10/22

Bibliographical note

Publisher Copyright:
© 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

3D Culturing
Hypoxia
Metastasis
Microfluidic Chip
Oxygen Gradient

Cite this

Zuo, P., Sleeboom, J. J. F., & den Toonder, J. M. J. (2022). 3D-Oxygen Gradient Chip for Cancer Cell Migration Research. In MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 514-515). (MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

Zuo, Pan ; Sleeboom, Jelle J.F. ; den Toonder, Jaap M.J. / 3D-Oxygen Gradient Chip for Cancer Cell Migration Research. MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2022. pp. 514-515 (MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

@inproceedings{dc6bef3cfeca43728e57d7ca661138d4,

title = "3D-Oxygen Gradient Chip for Cancer Cell Migration Research",

abstract = "Metastasis, the major cause of cancer related deaths, is a multi-step procedure, influenced by many factors and cues present in the tumor microenvironment. One important but not thoroughly studied factor is hypoxia. In this study, we introduce the design, fabrication and validation of a PDMS chip to study the effect of hypoxia on the migration of cancer cells in 3D. The chip contains a cell culture chamber in which a linear and stable oxygen gradient can be established by an oxygen leaching fluid flowing through a neighboring channel, and enables to observe the migration of cancer cells in 3D.",

keywords = "3D Culturing, Hypoxia, Metastasis, Microfluidic Chip, Oxygen Gradient",

author = "Pan Zuo and Sleeboom, \{Jelle J.F.\} and \{den Toonder\}, \{Jaap M.J.\}",

note = "Publisher Copyright: {\textcopyright} 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 ; Conference date: 23-10-2022 Through 27-10-2022",

year = "2022",

language = "English",

series = "MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

pages = "514--515",

booktitle = "MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

}

Zuo, P, Sleeboom, JJF & den Toonder, JMJ 2022, 3D-Oxygen Gradient Chip for Cancer Cell Migration Research. in MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 514-515, 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022, Hybrid, Hangzhou, China, 23/10/22.

3D-Oxygen Gradient Chip for Cancer Cell Migration Research. / Zuo, Pan (Corresponding author); Sleeboom, Jelle J.F.; den Toonder, Jaap M.J.
MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2022. p. 514-515 (MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - 3D-Oxygen Gradient Chip for Cancer Cell Migration Research

AU - Zuo, Pan

AU - Sleeboom, Jelle J.F.

AU - den Toonder, Jaap M.J.

PY - 2022

Y1 - 2022

N2 - Metastasis, the major cause of cancer related deaths, is a multi-step procedure, influenced by many factors and cues present in the tumor microenvironment. One important but not thoroughly studied factor is hypoxia. In this study, we introduce the design, fabrication and validation of a PDMS chip to study the effect of hypoxia on the migration of cancer cells in 3D. The chip contains a cell culture chamber in which a linear and stable oxygen gradient can be established by an oxygen leaching fluid flowing through a neighboring channel, and enables to observe the migration of cancer cells in 3D.

AB - Metastasis, the major cause of cancer related deaths, is a multi-step procedure, influenced by many factors and cues present in the tumor microenvironment. One important but not thoroughly studied factor is hypoxia. In this study, we introduce the design, fabrication and validation of a PDMS chip to study the effect of hypoxia on the migration of cancer cells in 3D. The chip contains a cell culture chamber in which a linear and stable oxygen gradient can be established by an oxygen leaching fluid flowing through a neighboring channel, and enables to observe the migration of cancer cells in 3D.

KW - 3D Culturing

KW - Hypoxia

KW - Metastasis

KW - Microfluidic Chip

KW - Oxygen Gradient

UR - https://www.scopus.com/pages/publications/85175714720

M3 - Conference contribution

AN - SCOPUS:85175714720

T3 - MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences

SP - 514

EP - 515

BT - MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022

Y2 - 23 October 2022 through 27 October 2022

ER -

Zuo P, Sleeboom JJF, den Toonder JMJ. 3D-Oxygen Gradient Chip for Cancer Cell Migration Research. In MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2022. p. 514-515. (MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

3D-Oxygen Gradient Chip for Cancer Cell Migration Research

Abstract

Publication series

Conference

Bibliographical note

UN SDGs

Keywords

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Cite this