• Groene Loper , Building 14, Helix (room STO 3.39)

    5612 AP Eindhoven

    Netherlands

  • P.O. Box 513, Department of Biomedical Engineering

    5600 MB Eindhoven

    Netherlands

Organization profile

Introduction / mission

At the protein engineering group we develop new proteins for applications in therapy and diagnosis, while critically testing our understanding of protein function.

Highlighted phrase

Beyond the already impressive functional diversity of natural proteins

Organisational profile

Our group operates at the interface of chemical biology and synthetic biology. Combining protein engineering and DNA nanotechnology, we develop intelligent biomolecular sensors and switches for applications in intracellular imaging, optogenetics, point-of-care diagnostics and antibody-based therapies. An important research theme is to develop generic engineering concepts for the development of protein-based switches, which include fluorescent sensors for intracellular imaging of metal ions, photo-switchable proteins, and protein-based sensors for antibody detection and actuation. We have an active policy of sharing our protein building blocks by depositing our plasmids to AddGene and promote their use by writing dedicated protocols and tutorial reviews. In education, our main goal is to train bachelor, masters and PhD students to become creative biomolecular engineers and independent critical scientists.

Fingerprint Dive into the research topics where Protein Engineering is active. These topic labels come from the works of this organisation's members. Together they form a unique fingerprint.

  • Network Recent external collaboration on country level. Dive into details by clicking on the dots.

    Research Output

    Continuous small-molecule monitoring with a digital single particle switch

    Yan, J., van Smeden, L., Merkx, M., Zijlstra, P. & Prins, M. W. J., 24 Apr 2020, In : ACS Sensors. 5, 4, p. 1168-1176 9 p.

    Research output: Contribution to journalArticleAcademicpeer-review

  • Dynamic modulation of proximity-induced enzyme activity using supramolecular polymers

    Magdalena Estirado, E., Rosier, B. J. H. M., de Greef, T. F. A. & Brunsveld, L., 22 Apr 2020, In : Chemical Communications.

    Research output: Contribution to journalArticleAcademicpeer-review

    Open Access
  • Engineered living materials based on adhesin-mediated trapping of programmable cells

    Guo, S., Dubuc, E., Rave, Y., Verhagen, M., Twisk, S. A. E., van der Hek, T., Oerlemans, G. J. M., van den Oetelaar, M. C. M., van Hazendonk, L. S., Brüls, M., Eijkens, B. V., Joostens, P. L., Keij, S. R., Xing, W., Nijs, M., Stalpers, J., Sharma, M., Gerth, M., Boonen, R. J. E. A., Verduin, K. & 3 others, Merkx, M., Voets, I. K. & De Greef, T. F. A., 20 Mar 2020, In : ACS Synthetic Biology. 9, 3, p. 475-485 11 p.

    Research output: Contribution to journalArticleAcademicpeer-review

    Open Access
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    Prizes

  • Exploring the diagnostics market for simple and fast point-of-care antibody detection

    M. Merkx (Recipient), 2013

    Prize: ERCProof of conceptScientific

  • NWO Vidi Award : Pieces of plastic with biological properties?

    P.Y.W. Dankers (Recipient), 2016

    Prize: NWOVidiScientific

  • Activities

    • 1 Editorial activity

    Nature Methods (Journal)

    Jan Vinkenborg (Editorial board member)
    2009

    Activity: Publication peer-review and editorial work typesEditorial activityScientific

    Research areas

    Bioluminescent sensors for point-of-care diagnostics

    Marjo van Hoof (Content manager), Lieke van Gorp (Content manager), Amy Broeders (Content manager)

    Impact: Research Topic/Theme (at group level)

    FRET and BRET sensors for intracellular imaging

    Marjo van Hoof (Content manager), Lieke van Gorp (Content manager), Amy Broeders (Content manager)

    Impact: Research Topic/Theme (at group level)

    Protein sensing and actuation using DNA-based molecular circuits

    Marjo van Hoof (Content manager), Lieke van Gorp (Content manager), Amy Broeders (Content manager)

    Impact: Research Topic/Theme (at group level)

    Student theses

    Benzene-1,3,5-tricarboxamides as supramolecular ordering moieties in polymeric materials

    Author: Mes, T., May 2007

    Supervisor: Palmans, A. (Supervisor 1), Roosma, J. (Supervisor 2) & Meijer, B. (Supervisor 2)

    Student thesis: Master

    Bioactivation of electrospun UPy-based supramolecular polymer meshes: towards an improved treatment of pelvic floor dysfunctions

    Author: Arts, B., 18 Dec 2017

    Supervisor: Dankers, P. (Supervisor 1), Zaccaria, S. (Supervisor 2) & Meijer, B. (Supervisor 2)

    Student thesis: Master

    Bioluminescent antibody conjugates for direct detection of analytes in solution

    Author: van Aalen, E., 28 Mar 2019

    Supervisor: Ni, Y. (Supervisor 1) & Merkx, M. (Supervisor 2)

    Student thesis: Master