High Performance Liquid Chromatography (HPLC)

Facility/equipment: Equipment

    Equipments Details

    Description

    Introduction

    HPLC is an analytical technique for the separation, identification and quantification of solutes in a solvent.

    Principle

    Differences in the relative affinities of molecules for the mobile phase and the stationary phase separate a sample into its constituents. The eluent can be a single solvent (isocratic) or a mixture with changing composition (gradient). There are three main operational modes: normal phase - based on polarity; reversed phase - based on hydrophobic interactions and ion exchange - based on electrostatic interactions.

    Applications

    • Identification and quantification and of solutes and components.
    • Micropollutant detection
    • Membrane performance evaluation

    Each component is associated with a peak characteristic retention time. The concentration is proportional to the integrated peak area.

    Details

    Name1100 and 1200 Series
    ManufacturersAgilent Technologies
    NameHPLC 1200 & 1250
    Photo associated with equipment - hplc_1.png
    Photo associated with equipment - hplc_2.png
    Photo associated with equipment - HPLC_1250_ON0_11.jpg

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