This data set contains the source data of the publication: Stef Broeren, Inês Figueiredo Pereira, Tongsheng Wang, Jaap den Toonder, Ye Wang. On-demand microfluidic mixing by actuating integrated magnetic microwalls. Lab on a Chip 2023,23, 1524-1530. DOI https://doi.org/10.1039/D2LC01168A. We demonstrated an on-demand micromixer based on the actuation of magnetic microwalls. The data are experimentally obtained with methods described in the publication.