Chemical biology

URL study guide

Description

• Small-molecule modulation of protein function
• Protein engineering
• Chemical genomics
• Expressed protein ligation and native chemical ligation
• In vivo protein labelling
• Activity-based protein profiling
• Phage display and yeast hybrids

Objectives

1. Different methods of protein structure elucidation (NMR, Xray), protein domain organization, protein engineering, peptide recognition, peptide synthesis, protein-ligand interactions and can describe them and their use in chemical biology.
2. Chemical biology compounds and can describe them and explain how cell surface and nuclear receptors can be analyzed by these compounds.
3. The concepts of microfluidic devices and macroscopic assay and can describe the advantages of microfluidic devices compared to macroscopic assays and explain methods of preparing these devices.
4. The concept of Activity-Based Protein Profiling (ABPP), the ‘bump-and-hole’ approach and can explain, describe and design specific enzyme-ligand pairs and different methods of protein labelling.
5. Different assay techniques and can describe and reflect on the different means of identifying bioactive molecules.
6. The concepts of DNA sequencing and RNA technologies and can discuss technological advances in DNA sequencing and the use of RNA technologies to modulate biological systems.

Method of Assessment